The effect of temperature on the oxygen-binding properties of hemoglobin (Hb) from ruminants, such as ox, reindeer, musk ox, mouflon and egyptian water buffalo is compared to that of human adult Hb (HbA). A striking difference emerges where in the presence of chloride ions and in the absence of 2,3-diphosphoglycerate [Gri(2,3)P2] a strongly reduced exothermic oxygenation process is observed for all ruminant Hb investigated with respect to HbA. Next, in the presence of physiological concentrations of Gri(2,3)P2, HbA displays a less exothermic oxygenation process, with values tending toward those observed in ruminant Hb [where Gri(2,3)P2 is not a physiological effector and for which the addition of Gri(2,3)P2 has essentially no effect on the oxygenation enthalpy]. Different from HbA, the intrinsically less exothermic oxygen binding seems to be independent of the experimental conditions for ruminant Hb, underlying specific structural characteristics which might be responsible for this feature.

Coletta, M., Clementi, M., Ascenzi, P., Petruzzelli, R., Condò, S., & Giardina, B. (1992). A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins. EUROPEAN JOURNAL OF BIOCHEMISTRY, 204(3), 1155-1157.

A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins

COLETTA, MASSIMILIANO;CONDO', SAVERIO GIOVANNI;
1992

Abstract

The effect of temperature on the oxygen-binding properties of hemoglobin (Hb) from ruminants, such as ox, reindeer, musk ox, mouflon and egyptian water buffalo is compared to that of human adult Hb (HbA). A striking difference emerges where in the presence of chloride ions and in the absence of 2,3-diphosphoglycerate [Gri(2,3)P2] a strongly reduced exothermic oxygenation process is observed for all ruminant Hb investigated with respect to HbA. Next, in the presence of physiological concentrations of Gri(2,3)P2, HbA displays a less exothermic oxygenation process, with values tending toward those observed in ruminant Hb [where Gri(2,3)P2 is not a physiological effector and for which the addition of Gri(2,3)P2 has essentially no effect on the oxygenation enthalpy]. Different from HbA, the intrinsically less exothermic oxygen binding seems to be independent of the experimental conditions for ruminant Hb, underlying specific structural characteristics which might be responsible for this feature.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
Animals; Mammals; Oxyhemoglobins; Thermodynamics; Humans; Hydrogen-Ion Concentration; Hemoglobin A; Chlorides; Diphosphoglyceric Acids; Temperature; Oxygen; 2,3-Diphosphoglycerate; Hemoglobins
Coletta, M., Clementi, M., Ascenzi, P., Petruzzelli, R., Condò, S., & Giardina, B. (1992). A comparative study of the temperature dependence of the oxygen-binding properties of mammalian hemoglobins. EUROPEAN JOURNAL OF BIOCHEMISTRY, 204(3), 1155-1157.
Coletta, M; Clementi, M; Ascenzi, P; Petruzzelli, R; Condo', Sg; Giardina, B
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/54687
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