Achondroplasia in mice is a recessive genetic disorder, characterized by disproportionate dwarfism with reduced bone growth. The cause of this chondrodystrophy is unknown. In this study normal and achondroplastic mouse chondrocytes were cultured in monolayer primary culture, their differentiation was verified by immunofluorescence and their growth was compared. The results showed that achondroplastic cells exhibited a higher proliferative activity than control cells of the same age, confirmed also by a thymidine incorporation assay. Furthermore, basic fibroblast growth factor treatment was found to induce a strong increase in growth of normal mouse chondrocytes, while it did not stimulate statistically significant proliferation of achondroplastic mouse cells. We suppose that this different growth rate could play a role in achondroplastic phenotype development.

Argentin G., C.R. (2000). In vitro proliferation of achondroplastic and normal mouse chondrocytes, before and after basic fibroblast growth factor stimulation. CELL PROLIFERATION, 33(6), 397-405 [10.1046/j.1365-2184.2000.00185.x].

In vitro proliferation of achondroplastic and normal mouse chondrocytes, before and after basic fibroblast growth factor stimulation

ARGENTIN, GABRIELLA;CICCHETTI, ROSADELE
2000

Abstract

Achondroplasia in mice is a recessive genetic disorder, characterized by disproportionate dwarfism with reduced bone growth. The cause of this chondrodystrophy is unknown. In this study normal and achondroplastic mouse chondrocytes were cultured in monolayer primary culture, their differentiation was verified by immunofluorescence and their growth was compared. The results showed that achondroplastic cells exhibited a higher proliferative activity than control cells of the same age, confirmed also by a thymidine incorporation assay. Furthermore, basic fibroblast growth factor treatment was found to induce a strong increase in growth of normal mouse chondrocytes, while it did not stimulate statistically significant proliferation of achondroplastic mouse cells. We suppose that this different growth rate could play a role in achondroplastic phenotype development.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
basic fibroblast growth factor; achondroplasia; animal cell; article; cartilage cell; cell growth; cell proliferation; controlled study; immunofluorescence; mouse; nonhuman; Achondroplasia; Animals; Cell Division; Cells, Cultured; Chondrocytes; DNA; Fibroblast Growth Factor 2; Mice; Mitogens; Thymidine; Animalia
Argentin G., C.R. (2000). In vitro proliferation of achondroplastic and normal mouse chondrocytes, before and after basic fibroblast growth factor stimulation. CELL PROLIFERATION, 33(6), 397-405 [10.1046/j.1365-2184.2000.00185.x].
Argentin, G; Cicchetti, R
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/54544
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