Two pesticides, organophosphate phosphamidon (PHO) and organochlorine dieldrin (DED) were assayed by the mouse bone marrow micronucleus test, to ascertain whether they showed genotoxic activity in vivo. Two doses, sub-lethal (PHO = 3 mg/kg b.wt.; DED = 60 mg/kg b.wt.) and lethal (PHO = 5 mg/kg b.wt.; DED = 90 mg/kg b.wt.), of each substance were administered intraperitoneally to 9-10-week old CBA male mice, in acute and repeated exposure. The sub-lethal dose was also administered at two different times and twice at 24-h intervals. Both PHO and DED proved able to induce a dose-dependent increase of micronucleated polychromatic erythrocytes (PCE). The two pesticides also showed a different detoxification time. Furthermore, the CREST staining with antikinetochore antibodies allowed us to conclude that the two chemicals are clastogens.

Cicchetti, R., Bari, M., Argentin, G. (1999). Induction of micronuclei in bone marrow by two pesticides and their differentiation with CREST staining: An in vivo study in mice. MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 439(2), 239-248 [10.1016/S1383-5718(98)00185-5].

Induction of micronuclei in bone marrow by two pesticides and their differentiation with CREST staining: An in vivo study in mice

CICCHETTI, ROSADELE;BARI, MONICA;ARGENTIN, GABRIELLA
1999-01-01

Abstract

Two pesticides, organophosphate phosphamidon (PHO) and organochlorine dieldrin (DED) were assayed by the mouse bone marrow micronucleus test, to ascertain whether they showed genotoxic activity in vivo. Two doses, sub-lethal (PHO = 3 mg/kg b.wt.; DED = 60 mg/kg b.wt.) and lethal (PHO = 5 mg/kg b.wt.; DED = 90 mg/kg b.wt.), of each substance were administered intraperitoneally to 9-10-week old CBA male mice, in acute and repeated exposure. The sub-lethal dose was also administered at two different times and twice at 24-h intervals. Both PHO and DED proved able to induce a dose-dependent increase of micronucleated polychromatic erythrocytes (PCE). The two pesticides also showed a different detoxification time. Furthermore, the CREST staining with antikinetochore antibodies allowed us to conclude that the two chemicals are clastogens.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
CREST staining; Dieldrin; Mouse bone marrow micronucleus test; Pesticide; Phosphamidon
Cicchetti, R., Bari, M., Argentin, G. (1999). Induction of micronuclei in bone marrow by two pesticides and their differentiation with CREST staining: An in vivo study in mice. MUTATION RESEARCH. GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 439(2), 239-248 [10.1016/S1383-5718(98)00185-5].
Cicchetti, R; Bari, M; Argentin, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/54540
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