We have determined the sites that are preferentially cleaved by Mn(T4MPyP) (where T4MPyP is the dianion of 5, 10, 15, 20, tetrakis (4-N-methylpyridine)porphyrin) on synthetic DNAs and on both intrinsically curved and average-shaped natural DNA sequences. On the basis of cleavage selectivity and of DNase I footprinting we show that the recognition specificity by this compound is based on steric properties: the preferred conformation is a DNA minor groove narrower than average and dimensionally defined. This conclusion is reached on the basis of: (i) the localization of the preferential cleavage sites at the 3' extremity of short A-tracts, known to undergo minor groove directional narrowing; (ii) the effects of temperature on cleavage specificity on curved sequences; (iii) the localization of cleavage sites in synthetic constructs whose crystal and solution structure was previously defined, and in programmed sequence variants thereoff; (iv) the effects of base substitutions on cleavage efficiency; (v) DNase I footprinting analysis. Several of these evidences argue against the possibility that Mn(T4MPyP)/DNA site selection occurs on the basis of electrostatic potential effects. Mn(T4MPyP) provides a tool for the analysis of DNA conformation whose selectivity is complementary to that of DNase I and hydroxyl radicals. (C) 1998 Academic Press.
|Tipologia:||Articolo su rivista|
|Citazione:||Di Mauro E., S.R. (1998). Manganese water-soluble porphyrin senses DNA conformation. JOURNAL OF MOLECULAR BIOLOGY, 282(1), 43-57.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore CHIM/03 - Chimica Generale e Inorganica|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||10.1006/jmbi.1998.1969|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1998|
|Titolo:||Manganese water-soluble porphyrin senses DNA conformation|
|Autori interni:||TAGLIATESTA, PIETRO|
|Autori:||Di Mauro E., Saladino R., Tagliatesta P., De Sanctis V., Negri R.|
|Appare nelle tipologie:||01 - Articolo su rivista|
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