This work reports the discovery and functional characterization of catalytically active hammerhead motifs within satellite DNA of the pDo500 family from several Dolichopoda cave cricket species. We show that in vitro transcribed RNA of some members of this satellite DNA family do self-cleave in vitro. This self-cleavage activity is correlated with the efficient in vivo processing of long primary transcripts into monomer-sized RNA. The high sequence conservation of the satellite pDo500 DNA family among genetically isolated Dolichopoda schiavazzii populations, as well as other Dolichopoda species, along with the fact that satellite members are actively transcribed in vivo suggests that the hammerhead-encoding satellite transcripts are under selective pressure, perhaps because they fulfil an important physiological role or function. Remarkably, this is the third example of hammerhead ribozyme structures associated with transcribed repetitive DNA sequences from animals. The possibility that such an association may not be purely coincidental is discussed.

Rojas, A.a., Vazquez Tello, A., Ferbeyre, G., Venanzetti, F., Bachmann, L., Paquin, B., et al. (2000). Hammerhead-mediated processing of satellite pDo500 family transcripts from Dolichopoda cave crickets. NUCLEIC ACIDS RESEARCH, 28(20), 4037-4043.

Hammerhead-mediated processing of satellite pDo500 family transcripts from Dolichopoda cave crickets

SBORDONI, VALERIO;
2000-01-01

Abstract

This work reports the discovery and functional characterization of catalytically active hammerhead motifs within satellite DNA of the pDo500 family from several Dolichopoda cave cricket species. We show that in vitro transcribed RNA of some members of this satellite DNA family do self-cleave in vitro. This self-cleavage activity is correlated with the efficient in vivo processing of long primary transcripts into monomer-sized RNA. The high sequence conservation of the satellite pDo500 DNA family among genetically isolated Dolichopoda schiavazzii populations, as well as other Dolichopoda species, along with the fact that satellite members are actively transcribed in vivo suggests that the hammerhead-encoding satellite transcripts are under selective pressure, perhaps because they fulfil an important physiological role or function. Remarkably, this is the third example of hammerhead ribozyme structures associated with transcribed repetitive DNA sequences from animals. The possibility that such an association may not be purely coincidental is discussed.
2000
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/05 - ZOOLOGIA
English
Con Impact Factor ISI
repetitive DNA; ribozyme; RNA; satellite DNA; article; DNA cleavage; DNA sequence; enzyme active site; enzyme activity; enzyme assay; enzyme conformation; enzyme isolation; enzyme structure; female; genetic conservation; insect; mouse; nonhuman; priority journal; protein processing; RNA transcription; species difference; Animals; Base Sequence; Catalysis; Conserved Sequence; Databases, Factual; DNA, Satellite; Gene Expression; Gryllidae; Kinetics; Magnesium Chloride; Models, Genetic; Multigene Family; Nucleic Acid Conformation; Point Mutation; RNA Processing, Post-Transcriptional; RNA, Catalytic; Software; Temperature; Transcription, Genetic; Animalia; Dolichopoda; Dolichopoda schiavazzii; Gryllidae; Insecta; Rhaphidophoridae
Rojas, A.a., Vazquez Tello, A., Ferbeyre, G., Venanzetti, F., Bachmann, L., Paquin, B., et al. (2000). Hammerhead-mediated processing of satellite pDo500 family transcripts from Dolichopoda cave crickets. NUCLEIC ACIDS RESEARCH, 28(20), 4037-4043.
Rojas, Aa; Vazquez Tello, A; Ferbeyre, G; Venanzetti, F; Bachmann, L; Paquin, B; Sbordoni, V; Cedergren, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53816
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