We present here the analysis of the genomic organization of the Fugu gene coding for ribosomal protein S3 and its intron encoded U15 RNA, and compare it with the homologous human and Xenopus genes. Only two of the six Fugu S3 gene introns do not contain the U15 sequence and are in fact shorter than 100 nucleotides, as most Fugu introns. The other four introns are somewhat longer and contain sequences homologous to U15 RNA; two of these represent functional copies, as shown by microinjections of Fugu transcripts into Xenopus oocytes, whereas the other two appear to be nonfunctional pseudocopies. Thus Fugu turns out to be ideal for the study of intron encoded snoRNAs, partly because of the reduced cloning and sequencing workload, and partly because the intron length per se can be an indication of the presence of a snoRNA coding sequence.

Crosio C., C.F. (1996). Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene. PCR METHODS AND APPLICATIONS, 6(12), 1227-1231.

Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene

CECCONI, FRANCESCO;CESARENI, GIOVANNI;AMALDI, FRANCESCO
1996

Abstract

We present here the analysis of the genomic organization of the Fugu gene coding for ribosomal protein S3 and its intron encoded U15 RNA, and compare it with the homologous human and Xenopus genes. Only two of the six Fugu S3 gene introns do not contain the U15 sequence and are in fact shorter than 100 nucleotides, as most Fugu introns. The other four introns are somewhat longer and contain sequences homologous to U15 RNA; two of these represent functional copies, as shown by microinjections of Fugu transcripts into Xenopus oocytes, whereas the other two appear to be nonfunctional pseudocopies. Thus Fugu turns out to be ideal for the study of intron encoded snoRNAs, partly because of the reduced cloning and sequencing workload, and partly because the intron length per se can be an indication of the presence of a snoRNA coding sequence.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/06
English
Con Impact Factor ISI
ribosome protein; animal cell; article; codon; controlled study; fish; gene sequence; gene structure; human; human cell; intron; molecular cloning; nonhuman; nucleotide sequence; oocyte; priority journal; sequence homology; xenopus; Animals; Base Sequence; DNA, Complementary; Fishes, Poisonous; Humans; Introns; Molecular Sequence Data; Ribosomal Proteins; RNA, Small Nuclear
Crosio C., C.F. (1996). Fugu intron oversize reveals the presence of U15 snoRNA coding sequences in some introns of the ribosomal protein S3 gene. PCR METHODS AND APPLICATIONS, 6(12), 1227-1231.
Crosio, C; Cecconi, F; Mariottini, P; Cesareni, G; Brenner, S; Amaldi, F
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/53617
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