[125I]-[D-Ala2]-beta EP*) binding to Epstein-Barr Virus (EBV) transformed B lymphocytes or to freshly isolated lymphocytes was characterized. The binding was time-, temperature- and pH-dependent; furthermore, it was reversible and cell concentration dependent. Maximum binding appeared at 15C in Tris buffer (pH 7.6) containing both BSA 0.5% and Bacitracin 0.1 mg/ml. beta EP inhibited BEP* binding to transformed lymphocytes and to freshly isolated lymphocytes by 50% at approximately 4 x 10(-8) M and 8 x 10(-9) M, respectively. Peptides representing amino-acid sequences 1-5, 1-16 and 1-17 of beta EP did not inhibit beta EP binding, neither did the opiates compounds Naloxone, Morphine, Bremazocine and Ethylketocyclazocine. On the contrary, beta EPd (6-31) inhibited beta EP* binding as effectively as beta EP, thus indicating that beta EP* binding to lymphocytes does not involve the N-terminal region of beta EP. 2 x 10(7)/ml freshly isolated lymphocytes bound 1.53 +/- 75%, whereas 2 x 10(6)/ml transformed lymphocytes bound 1.64 +/- 0.75% (mean +/- SD) beta EP* at tracer concentration. EBV transformed lymphocytes and freshly isolated lymphocytes may be useful to study beta EP receptors in humans.
Borboni, P., Di Cola, G., Sesti, G., Marini, M., Del Porto, P., Gilardini Montani, M., et al. (1989). Beta-endorphin receptors on cultured and freshly isolated lymphocytes from normal subjects. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 163(1), 642-8.
Tipologia: | Articolo su rivista |
Citazione: | Borboni, P., Di Cola, G., Sesti, G., Marini, M., Del Porto, P., Gilardini Montani, M., et al. (1989). Beta-endorphin receptors on cultured and freshly isolated lymphocytes from normal subjects. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 163(1), 642-8. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore MED/09 - Medicina Interna |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 30-ago-1989 |
Titolo: | Beta-endorphin receptors on cultured and freshly isolated lymphocytes from normal subjects |
Autori: | |
Autori: | Borboni, P; Di Cola, G; Sesti, G; Marini, M; Del Porto, P; Gilardini Montani, M; Lauro, R; De Pirro, R |
Appare nelle tipologie: | 01 - Articolo su rivista |