The human insulin receptor gene is expressed in two variant isoforms which differ by the absence (HIR-A) or presence (HIR-B) of 12 amino acids in the COOH-terminus of the extracellular alpha-subunit as a consequence of alternative splicing of exon 11. Expression of the two variant isoforms is regulated in a tissue-specific manner. In this study, we have measured the levels of the two receptor variants in isolated adipocytes from 10 non-insulin-dependent diabetes mellitus (NIDDM) and 11 normal subjects using an immunological assay, based on the ability of a human anti-receptor autoantibody to discriminate between HIR-A and HIR-B. Results indicate that levels of HIR-B variant are increased in NIDDM patients.

Sesti, G., Marini, M.a., Tullio, A., Montemurro, A., Borboni, P., Fusco, A., et al. (1991). Altered expression of the two naturally occurring human insulin receptor variants in isolated adipocytes of non-insulin-dependent diabetes mellitus patients. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 181(3), 1419-24.

Altered expression of the two naturally occurring human insulin receptor variants in isolated adipocytes of non-insulin-dependent diabetes mellitus patients

MARINI, MARIA ADELAIDE;MONTEMURRO, ANTONIO;BORBONI, PATRIZIA;LAURO, RENATO
1991-12-31

Abstract

The human insulin receptor gene is expressed in two variant isoforms which differ by the absence (HIR-A) or presence (HIR-B) of 12 amino acids in the COOH-terminus of the extracellular alpha-subunit as a consequence of alternative splicing of exon 11. Expression of the two variant isoforms is regulated in a tissue-specific manner. In this study, we have measured the levels of the two receptor variants in isolated adipocytes from 10 non-insulin-dependent diabetes mellitus (NIDDM) and 11 normal subjects using an immunological assay, based on the ability of a human anti-receptor autoantibody to discriminate between HIR-A and HIR-B. Results indicate that levels of HIR-B variant are increased in NIDDM patients.
31-dic-1991
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/09 - MEDICINA INTERNA
English
Con Impact Factor ISI
Male; 3T3 Cells; Middle Aged; Transfection; Female; Genetic Variation; Animals; Diabetes Mellitus, Type 2; Humans; Kinetics; Gene Expression; Insulin; Immunoglobulin G; Macromolecular Substances; Receptor, Insulin; Mice; Adipose Tissue; Amino Acid Sequence; Reference Values
Sesti, G., Marini, M.a., Tullio, A., Montemurro, A., Borboni, P., Fusco, A., et al. (1991). Altered expression of the two naturally occurring human insulin receptor variants in isolated adipocytes of non-insulin-dependent diabetes mellitus patients. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 181(3), 1419-24.
Sesti, G; Marini, Ma; Tullio, A; Montemurro, A; Borboni, P; Fusco, A; Accili, D; Lauro, R
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53377
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 49
  • ???jsp.display-item.citation.isi??? ND
social impact