Human mast cells (HMC-1) take up anandamide (arachidonoyl-ethanolamide. AEA) with a saturable process (K-m = 200 +/- 20 nM, V-max = 25 +/- 3 pmol min(-1) mg protein(-1)), enhanced two-fold over control by nitric oxide-donors. Internalized AEA was hydrolized by a fatty acid amide hydrolase (FAAK), whose activity became measurable only in the presence of 5-lipoxygenase, but not cyclooxygenase, inhibitors. FAAH (K-m = 5.0 +/- 0.5 mu M, V-max = 160 +/- 15 pmol min(-1) mg protein(-1)) was competitively inhibited by palmitoylethanolamide. HMC-1 cells did not display a functional cannabinoid receptor on their surface and neither. AEA nor palmitoylethanolamide affected tryptase release from these cells. (C) 2000 Federation of European Biochemical Societies.

Maccarrone, M., Fiorucci, L., Erba, F., Bari, M., Finazzi Agro, A., Ascoli, F. (2000). Human mast cells take up and hydrolyze anandamide under the control of 5-lipoxygenase and do not express cannabinoid receptors. FEBS LETTERS, 468, 176-180 [10.1016/S0014-5793(00)01223-0].

Human mast cells take up and hydrolyze anandamide under the control of 5-lipoxygenase and do not express cannabinoid receptors

FIORUCCI, LAURA;ERBA, FULVIO;
2000-01-01

Abstract

Human mast cells (HMC-1) take up anandamide (arachidonoyl-ethanolamide. AEA) with a saturable process (K-m = 200 +/- 20 nM, V-max = 25 +/- 3 pmol min(-1) mg protein(-1)), enhanced two-fold over control by nitric oxide-donors. Internalized AEA was hydrolized by a fatty acid amide hydrolase (FAAK), whose activity became measurable only in the presence of 5-lipoxygenase, but not cyclooxygenase, inhibitors. FAAH (K-m = 5.0 +/- 0.5 mu M, V-max = 160 +/- 15 pmol min(-1) mg protein(-1)) was competitively inhibited by palmitoylethanolamide. HMC-1 cells did not display a functional cannabinoid receptor on their surface and neither. AEA nor palmitoylethanolamide affected tryptase release from these cells. (C) 2000 Federation of European Biochemical Societies.
2000
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Anandamide; Endocannabinoid; Inflammation; Lipoxygenase; Nitric oxide; Tryptase
Maccarrone, M., Fiorucci, L., Erba, F., Bari, M., Finazzi Agro, A., Ascoli, F. (2000). Human mast cells take up and hydrolyze anandamide under the control of 5-lipoxygenase and do not express cannabinoid receptors. FEBS LETTERS, 468, 176-180 [10.1016/S0014-5793(00)01223-0].
Maccarrone, M; Fiorucci, L; Erba, F; Bari, M; Finazzi Agro, A; Ascoli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53352
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