Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is induced by copper and silver, as expected on the basis of previous results indicating that ACE1 binds only Ag(I) besides Cu(I). We conclude that at the transcriptional level Cu,ZnSOD is co-regulated with metallothionein. Furthermore, structural similarities between the two promoters were found, which could explain the co-regulation effect and the quantitative differences in the response of the two genes to copper.

Carri', M.t., Galiazzo, F., Ciriolo, M.r., Rotilio, G. (1991). Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor. FEBS LETTERS, 278(2), 263-266.

Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor

CARRI', MARIA TERESA;CIRIOLO, MARIA ROSA;ROTILIO, GIUSEPPE
1991-01-28

Abstract

Saccharomyces cerevisiae mutant strain DTY26, lacking ACE1, the protein mediator for the induction of metallothionein gene expression, is unable to increase Cu,Zn superoxide dismutase mRNA in response to copper. In the wild-type strain DTY22 transcription of both Cu,Zn superoxide dismutase and metallothionein genes is induced by copper and silver, as expected on the basis of previous results indicating that ACE1 binds only Ag(I) besides Cu(I). We conclude that at the transcriptional level Cu,ZnSOD is co-regulated with metallothionein. Furthermore, structural similarities between the two promoters were found, which could explain the co-regulation effect and the quantitative differences in the response of the two genes to copper.
28-gen-1991
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Gene Expression Regulation, Fungal; Superoxide Dismutase; DNA, Fungal; Blotting, Northern; Metallothionein; Sequence Homology, Nucleic Acid; DNA-Binding Proteins; Saccharomyces cerevisiae Proteins; RNA, Fungal; Transcription, Genetic; Copper; Saccharomyces cerevisiae; RNA, Messenger; Base Sequence; Promoter Regions, Genetic; Transcription Factors; Molecular Sequence Data; Silver
Carri', M.t., Galiazzo, F., Ciriolo, M.r., Rotilio, G. (1991). Evidence for co-regulation of Cu,Zn superoxide dismutase and metallothionein gene expression in yeast through transcriptional control by copper via the ACE 1 factor. FEBS LETTERS, 278(2), 263-266.
Carri', Mt; Galiazzo, F; Ciriolo, Mr; Rotilio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53351
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