We show that thiol-groups confer redox-susceptibility to the zinc-finger transcription factor Sp1 and that this redox-susceptibility is prevented by DNA-binding and depends on zinc-coordination of the protein. Apo-Sp1 contained in metal depleted nuclear extracts of human K562 cells exhibited a markedly increased susceptibility towards oxidizing and alkylating agents, as compared to holo-Sp 1. Moreover, DNA binding of apo-Sp1, but not of the holo-protein, was dramatically decreased in the presence of GSH/GSSG ratios within the physiological range. We compared these results with the redox behaviour of two other transcription factors, OTF-1 and NF1, which was found to be different in several aspects from that of Sp1.
Knoepfel, L., Steinkühler, C., Carri', M.t., Rotilio, G. (1994). Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 201(2), 871-877 [10.1006/bbrc.1994.1782].
Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1
CARRI', MARIA TERESA;ROTILIO, GIUSEPPE
1994-06-15
Abstract
We show that thiol-groups confer redox-susceptibility to the zinc-finger transcription factor Sp1 and that this redox-susceptibility is prevented by DNA-binding and depends on zinc-coordination of the protein. Apo-Sp1 contained in metal depleted nuclear extracts of human K562 cells exhibited a markedly increased susceptibility towards oxidizing and alkylating agents, as compared to holo-Sp 1. Moreover, DNA binding of apo-Sp1, but not of the holo-protein, was dramatically decreased in the presence of GSH/GSSG ratios within the physiological range. We compared these results with the redox behaviour of two other transcription factors, OTF-1 and NF1, which was found to be different in several aspects from that of Sp1.Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons