We show that thiol-groups confer redox-susceptibility to the zinc-finger transcription factor Sp1 and that this redox-susceptibility is prevented by DNA-binding and depends on zinc-coordination of the protein. Apo-Sp1 contained in metal depleted nuclear extracts of human K562 cells exhibited a markedly increased susceptibility towards oxidizing and alkylating agents, as compared to holo-Sp 1. Moreover, DNA binding of apo-Sp1, but not of the holo-protein, was dramatically decreased in the presence of GSH/GSSG ratios within the physiological range. We compared these results with the redox behaviour of two other transcription factors, OTF-1 and NF1, which was found to be different in several aspects from that of Sp1.

Knoepfel, L., Steinkühler, C., Carri', M.t., Rotilio, G. (1994). Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 201(2), 871-877 [10.1006/bbrc.1994.1782].

Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1

CARRI', MARIA TERESA;ROTILIO, GIUSEPPE
1994-06-15

Abstract

We show that thiol-groups confer redox-susceptibility to the zinc-finger transcription factor Sp1 and that this redox-susceptibility is prevented by DNA-binding and depends on zinc-coordination of the protein. Apo-Sp1 contained in metal depleted nuclear extracts of human K562 cells exhibited a markedly increased susceptibility towards oxidizing and alkylating agents, as compared to holo-Sp 1. Moreover, DNA binding of apo-Sp1, but not of the holo-protein, was dramatically decreased in the presence of GSH/GSSG ratios within the physiological range. We compared these results with the redox behaviour of two other transcription factors, OTF-1 and NF1, which was found to be different in several aspects from that of Sp1.
15-giu-1994
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Oligodeoxyribonucleotides; Glutathione; Humans; Cell Nucleus; Glutathione Disulfide; Sp1 Transcription Factor; Oxidation-Reduction; Ethylmaleimide; Base Sequence; Tumor Cells, Cultured; Kinetics; Zinc; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Molecular Sequence Data; Apoproteins; Cell Line
Knoepfel, L., Steinkühler, C., Carri', M.t., Rotilio, G. (1994). Role of zinc-coordination and of the glutathione redox couple in the redox susceptibility of human transcription factor Sp1. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 201(2), 871-877 [10.1006/bbrc.1994.1782].
Knoepfel, L; Steinkühler, C; Carri', Mt; Rotilio, G
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53340
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