The gene encoding for polynucleotide phosphorylase (pnp) of a new biovar of Staphylococcus aureus subsp. aureus (NBSA) has been isolated From a genomic library of strain M280(0), The coding region consisted of a 1094-bp HindIII-HindIII DNA fragment encoding for a protein of 277 amino acids with a calculated molecular mass of 29.5 kDa. The nucleotide sequence of the structural gene, contained a continuous open reading frame of 836 bp, showed significant homology with the genes of bacterial polynucleotide phosphorylase from Bacillus subtilis (67.7% identity), from Haemophilus influenzae (62.47% identity), from Pseudomonas luminescens (61.6% identity), and from Escherichia coli (59.7% identity). DNA-DNA and DNA-colony slot-blot hybridizations demonstrated that the pnp gene, employed as a molecular probe, is specific for the identification of NBSA strains. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Fontana, C., Favaro, M., Frezza, D., Favalli, C. (1999). Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar. FEMS MICROBIOLOGY LETTERS, 172(1), 47-52 [10.1016/S0378-1097(99)00004-X].

Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar

FONTANA, CARLA;FAVARO, MARCO;FREZZA, DOMENICO;FAVALLI, CARTESIO
1999-01-01

Abstract

The gene encoding for polynucleotide phosphorylase (pnp) of a new biovar of Staphylococcus aureus subsp. aureus (NBSA) has been isolated From a genomic library of strain M280(0), The coding region consisted of a 1094-bp HindIII-HindIII DNA fragment encoding for a protein of 277 amino acids with a calculated molecular mass of 29.5 kDa. The nucleotide sequence of the structural gene, contained a continuous open reading frame of 836 bp, showed significant homology with the genes of bacterial polynucleotide phosphorylase from Bacillus subtilis (67.7% identity), from Haemophilus influenzae (62.47% identity), from Pseudomonas luminescens (61.6% identity), and from Escherichia coli (59.7% identity). DNA-DNA and DNA-colony slot-blot hybridizations demonstrated that the pnp gene, employed as a molecular probe, is specific for the identification of NBSA strains. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/18 - Genetica
English
Con Impact Factor ISI
Hybridization probe; Molecular cloning; Sequence; Staphylococcus spp.
Fontana, C., Favaro, M., Frezza, D., Favalli, C. (1999). Isolation and characterization of a DNA probe for Staphylococcus aureus subspecies aureus biovar. FEMS MICROBIOLOGY LETTERS, 172(1), 47-52 [10.1016/S0378-1097(99)00004-X].
Fontana, C; Favaro, M; Frezza, D; Favalli, C
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53270
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