A highly spread polymorphism flanking the 3 C alpha 1 human IG heavy chain gene was identified. This polymorphism allowed the detection of an internal duplication within the 3' flanking region of both C alpha 1 and C alpha 2. This region has a regulatory function with four enhancer structures also present at the 3' end of the human C alpha 2 as well as in that of mouse and rat single C alpha genes. The 5682-bp sequence of clone lambda p18 described here starts 3' of C alpha 1 and presents three open reading frames; one of them contains part of the tandem repeats with the 20-bp consensus described previously that is expressed in a poly(A)(+) RNA and found in three dbEST clones of the human tonsillar cDNA library. Here, we demonstrate that in the CLF1 B lymphoblastoid cell line, this transcript is associated with polysomes. We also discuss the possibility of the presence of a new regulatory gene that does not encode an immunoglobulin and maps in the human IG heavy chain gene cluster. (C) 1998 Elsevier Science B.V. All rights reserved.

Frezza D., C.O. (1998). The region 3 ' to C alpha 1 gene of human IG heavy chain displays a polymorphic duplicated sequence and encodes an RNA associated with polysomes. GENE, 219(2009/02/01 00:00:00.000), 19-24 [10.1016/S0378-1119(98)00399-0].

The region 3 ' to C alpha 1 gene of human IG heavy chain displays a polymorphic duplicated sequence and encodes an RNA associated with polysomes

FREZZA, DOMENICO;FAVARO, MARCO;
1998

Abstract

A highly spread polymorphism flanking the 3 C alpha 1 human IG heavy chain gene was identified. This polymorphism allowed the detection of an internal duplication within the 3' flanking region of both C alpha 1 and C alpha 2. This region has a regulatory function with four enhancer structures also present at the 3' end of the human C alpha 2 as well as in that of mouse and rat single C alpha genes. The 5682-bp sequence of clone lambda p18 described here starts 3' of C alpha 1 and presents three open reading frames; one of them contains part of the tandem repeats with the 20-bp consensus described previously that is expressed in a poly(A)(+) RNA and found in three dbEST clones of the human tonsillar cDNA library. Here, we demonstrate that in the CLF1 B lymphoblastoid cell line, this transcript is associated with polysomes. We also discuss the possibility of the presence of a new regulatory gene that does not encode an immunoglobulin and maps in the human IG heavy chain gene cluster. (C) 1998 Elsevier Science B.V. All rights reserved.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/18 - Genetica
English
Con Impact Factor ISI
20-bp tandem repeats; IG regulatory region; C alpha 1-C alpha 2 duplication; GA repeats
Frezza D., C.O. (1998). The region 3 ' to C alpha 1 gene of human IG heavy chain displays a polymorphic duplicated sequence and encodes an RNA associated with polysomes. GENE, 219(2009/02/01 00:00:00.000), 19-24 [10.1016/S0378-1119(98)00399-0].
Frezza, D; Camacho Vanegas, O; Fruscalzo, A; Favaro, M; Giorgi, S; D'Abusco, As; Gualandi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/53269
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