DNA synthesis of human hepatoblastoma HepG2 cells is reversibly inhibited by butyrate. When butyrate is removed from the culture medium, cells re-enter the cell cycle, synthesizing DNA with a time lag of about 12 h. HepG2 cells, growth-inhibited for 30 h with butyrate, synthesize and accumulate a nuclear protein, called D. Protein D synthesis is inhibited in cells which, released from the butyrate block, have resumed DNA synthesis as well as in growing cells never exposed to butyrate. Protein D has been purified from growth-arrested cells and partially sequenced. The amino acid sequences of five internal trypsin peptides indicate that protein D is a novel nuclear protein.

Donadel, G., Garzelli, C., Frank, R., Gabrielli, F. (1991). Identification of a novel nuclear protein synthesized in growth-arrested human hepatoblastoma HepG2 cells. EUROPEAN JOURNAL OF BIOCHEMISTRY, 195(3), 723-729 [10.1111/j.1432-1033.1991.tb15759.x].

Identification of a novel nuclear protein synthesized in growth-arrested human hepatoblastoma HepG2 cells

DONADEL, GIULIA;
1991-02-14

Abstract

DNA synthesis of human hepatoblastoma HepG2 cells is reversibly inhibited by butyrate. When butyrate is removed from the culture medium, cells re-enter the cell cycle, synthesizing DNA with a time lag of about 12 h. HepG2 cells, growth-inhibited for 30 h with butyrate, synthesize and accumulate a nuclear protein, called D. Protein D synthesis is inhibited in cells which, released from the butyrate block, have resumed DNA synthesis as well as in growing cells never exposed to butyrate. Protein D has been purified from growth-arrested cells and partially sequenced. The amino acid sequences of five internal trypsin peptides indicate that protein D is a novel nuclear protein.
Pubblicato
Rilevanza internazionale
Articolo
Esperti anonimi
Settore MED/04 - Patologia Generale
English
Butyric Acid; Carcinoma, Hepatocellular; Humans; Sulfur Radioisotopes; Amino Acid Sequence; Autoradiography; Methionine; Liver Neoplasms; Peptide Fragments; Nuclear Proteins; Kinetics; Electrophoresis, Gel, Two-Dimensional; Molecular Sequence Data; Butyric Acids; Cell Cycle; Cell Line; DNA Replication; Cell Division
Donadel, G., Garzelli, C., Frank, R., Gabrielli, F. (1991). Identification of a novel nuclear protein synthesized in growth-arrested human hepatoblastoma HepG2 cells. EUROPEAN JOURNAL OF BIOCHEMISTRY, 195(3), 723-729 [10.1111/j.1432-1033.1991.tb15759.x].
Donadel, G; Garzelli, C; Frank, R; Gabrielli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53217
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