This paper reports the optimisation of a competitive immunoassay (ELISA) to detect lactosylated proteins in milk samples. The assay employs monoclonal antibodies for lactosylated proteins produced in our laboratory and requires no pre-treatment of the samples other than a dilution step Monoclonal antibodies were fully characterised in terms of selectivity and cross-reactivity with structurally related molecules and used in a competitive assay format with lactosylated standard proteins (lactosylated ovalbumin). The detection limit for lactosylated ovalbumin was 0.015 mug ml(-1) and the working range was from 0.010 to 40 mug ml(-1). The data obtained indicate that the ELISA developed is applicable to diluted milk samples and is able to distinguish between milk samples that have undergone different heat treatments (UHT and pasteurised milk).

Pallini, M., Compagnone, D., Di Stefano, S., Marini, S., Coletta, M., & Palleschi, G. (2001). Immunodetection of lactosylated proteins as a useful tool to determine heat treatment in milk samples. ANALYST, 126(1), 66-70 [10.1039/b006482n].

Immunodetection of lactosylated proteins as a useful tool to determine heat treatment in milk samples

MARINI, STEFANO;COLETTA, MASSIMILIANO;PALLESCHI, GIUSEPPE
2001

Abstract

This paper reports the optimisation of a competitive immunoassay (ELISA) to detect lactosylated proteins in milk samples. The assay employs monoclonal antibodies for lactosylated proteins produced in our laboratory and requires no pre-treatment of the samples other than a dilution step Monoclonal antibodies were fully characterised in terms of selectivity and cross-reactivity with structurally related molecules and used in a competitive assay format with lactosylated standard proteins (lactosylated ovalbumin). The detection limit for lactosylated ovalbumin was 0.015 mug ml(-1) and the working range was from 0.010 to 40 mug ml(-1). The data obtained indicate that the ELISA developed is applicable to diluted milk samples and is able to distinguish between milk samples that have undergone different heat treatments (UHT and pasteurised milk).
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
milk protein; monoclonal antibody; ovalbumin; antibody specificity; article; cross reaction; enzyme linked immunosorbent assay; immunoassay; pasteurization; protein analysis; protein purification; Animals; Antibodies, Monoclonal; Enzyme-Linked Immunosorbent Assay; Female; Food Preservation; Heat; Lactulose; Mice; Mice, Inbred BALB C; Milk; Spectrophotometry
Pallini, M., Compagnone, D., Di Stefano, S., Marini, S., Coletta, M., & Palleschi, G. (2001). Immunodetection of lactosylated proteins as a useful tool to determine heat treatment in milk samples. ANALYST, 126(1), 66-70 [10.1039/b006482n].
Pallini, M; Compagnone, D; Di Stefano, S; Marini, S; Coletta, M; Palleschi, G
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/53100
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