We produced monoclonal antibodies against the coenzyme pyrrolequinoline quinone (PQQ). These antibodies were obtained by immunizing mice with PQQ conjugated to a chemically modified polypeptide in order to induce a strong immune response. Among the various antibodies obtained, one was found to bind (besides PQQ and 6-hydroxydopamine conjugated to carrier proteins) several different quinoenzymes, namely lentil seedling and bovine serum diamine oxidases and methylamine dehydrogenase. This antibody was able to inhibit the catalytic activity of these enzymes. Moreover, the monoclonal antibody recognized different proteins of lentil seeds on Western blots. Even the variable fragment of immunoglobulin heavy chains of this monoclonal antibody expressed in Escherichia coli is able to recognize the active site of different quinoenzymes.

Marini, S., Giardina, B., Fasciglione, G., FINAZZI AGRO', A. (1993). MONOCLONAL-ANTIBODY RECOGNIZES DIFFERENT QUINONE MOIETIES IN ENZYMES. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 268(18), 13352-13355.

MONOCLONAL-ANTIBODY RECOGNIZES DIFFERENT QUINONE MOIETIES IN ENZYMES

MARINI, STEFANO;FASCIGLIONE, GIOVANNI;FINAZZI AGRO', ALESSANDRO
1993-01-01

Abstract

We produced monoclonal antibodies against the coenzyme pyrrolequinoline quinone (PQQ). These antibodies were obtained by immunizing mice with PQQ conjugated to a chemically modified polypeptide in order to induce a strong immune response. Among the various antibodies obtained, one was found to bind (besides PQQ and 6-hydroxydopamine conjugated to carrier proteins) several different quinoenzymes, namely lentil seedling and bovine serum diamine oxidases and methylamine dehydrogenase. This antibody was able to inhibit the catalytic activity of these enzymes. Moreover, the monoclonal antibody recognized different proteins of lentil seeds on Western blots. Even the variable fragment of immunoglobulin heavy chains of this monoclonal antibody expressed in Escherichia coli is able to recognize the active site of different quinoenzymes.
1993
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
PLASMA AMINE OXIDASE; PYRROLOQUINOLINE QUINONE; METHYLAMINE DEHYDROGENASE; ACTIVE-SITE; PURIFICATION; 6-HYDROXYDOPA; COFACTOR; ASSAY
Marini, S., Giardina, B., Fasciglione, G., FINAZZI AGRO', A. (1993). MONOCLONAL-ANTIBODY RECOGNIZES DIFFERENT QUINONE MOIETIES IN ENZYMES. THE JOURNAL OF BIOLOGICAL CHEMISTRY, 268(18), 13352-13355.
Marini, S; Giardina, B; Fasciglione, G; FINAZZI AGRO', A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/53072
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