Non-classical antigen-presentation by CD1 molecules expressed on cytokine-activated monocytes (CAM), and cell-mediated responses supported by double-negative (DN) and by CD8(+) responder alpha beta T cells, are involved in host resistance against mycobacterial infections. The CD1b protein is responsible for presentation of non-peptide, lipid antigens to T cells. In this context, a pivotal role is played by induction of CD1b protein on the membrane of human monocytes activated by GM-CSF alone, and more efficiently by GM-CSF combined with IL-4. Rifampin (RFP), a drug which is extensively utilized for chemoprophylaxis or treatment of Mycobacterium tuberculosis, is known to reduce a number of B, or T cell-dependent responses. Therefore we undertook immunopharmacological studies on RFP, to determine the effects of this agent on human macrophage function, relative to antigen presentation by CD1b molecules and on DN T cell cytolytic function. The results showed that: (a) graded concentration of RFP (2 or 10 mu g/ml) induced a significant increase of CD1 expression, in CAM as evaluated by FAGS analysis;.(b) RFP increased significantly the specific mAb binding to CD1 on CAM surface; (c) treatment of effector cells with RFP did not reduce DN T cell-mediated cytolysis against lymphoblastoid cells transfected with CD1b cDNA (C1R.b6 cells), pulsed with IM. tuberculosis. These results suggest that RFP could be of potential value in improving mycobacterial antigen presentation without impairing responder T cell function.

Giuliani, A., Porcelli, S., Tentori, L., Graziani, G., Testorelli, C., Prete, S., et al. (1998). Effect of rifampin on CD1b expression and double-negative T cell responses against mycobacteria-derived glycolipid antigen. LIFE SCIENCES, 63(12), 985-994 [10.1016/S0024-3205(98)00360-9].

Effect of rifampin on CD1b expression and double-negative T cell responses against mycobacteria-derived glycolipid antigen

TENTORI, LUCIO;GRAZIANI, GRAZIA;BONMASSAR, ENZO;AQUINO, ANGELO
1998-01-01

Abstract

Non-classical antigen-presentation by CD1 molecules expressed on cytokine-activated monocytes (CAM), and cell-mediated responses supported by double-negative (DN) and by CD8(+) responder alpha beta T cells, are involved in host resistance against mycobacterial infections. The CD1b protein is responsible for presentation of non-peptide, lipid antigens to T cells. In this context, a pivotal role is played by induction of CD1b protein on the membrane of human monocytes activated by GM-CSF alone, and more efficiently by GM-CSF combined with IL-4. Rifampin (RFP), a drug which is extensively utilized for chemoprophylaxis or treatment of Mycobacterium tuberculosis, is known to reduce a number of B, or T cell-dependent responses. Therefore we undertook immunopharmacological studies on RFP, to determine the effects of this agent on human macrophage function, relative to antigen presentation by CD1b molecules and on DN T cell cytolytic function. The results showed that: (a) graded concentration of RFP (2 or 10 mu g/ml) induced a significant increase of CD1 expression, in CAM as evaluated by FAGS analysis;.(b) RFP increased significantly the specific mAb binding to CD1 on CAM surface; (c) treatment of effector cells with RFP did not reduce DN T cell-mediated cytolysis against lymphoblastoid cells transfected with CD1b cDNA (C1R.b6 cells), pulsed with IM. tuberculosis. These results suggest that RFP could be of potential value in improving mycobacterial antigen presentation without impairing responder T cell function.
1998
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14 - FARMACOLOGIA
English
Con Impact Factor ISI
antigen; glycolipid; granulocyte macrophage colony stimulating factor; antigen binding; antigen presenting cell; article; cell function; chemoprophylaxis; fluorescence activated cell sorter; host resistance; human; human cell; immune response; monocyte; mycobacterium tuberculosis; protein analysis; tuberculosis; antibiotics, antitubercular; antigens, CD1; cell adhesion; cell line; cell membrane; cytotoxicity, immunologic; flow cytometry; fluorescent antibody technique, direct; glycolipids; humans; indicators and reagents; rifampin; T-lymphocytes
Giuliani, A., Porcelli, S., Tentori, L., Graziani, G., Testorelli, C., Prete, S., et al. (1998). Effect of rifampin on CD1b expression and double-negative T cell responses against mycobacteria-derived glycolipid antigen. LIFE SCIENCES, 63(12), 985-994 [10.1016/S0024-3205(98)00360-9].
Giuliani, A; Porcelli, S; Tentori, L; Graziani, G; Testorelli, C; Prete, S; Bussini, S; Cappelletti, D; Brenner, M; Bonmassar, E; Aquino, A
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/52884
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