Strong immunogenicity is induced by antitumor triazene compounds in tumor cells through a mutagenic mechanism. A highly immunogenic 'D' clone, isolated from a dacarbazine-treated L5178Y leukemia of DBA/2 mice, was transfected with K-ras mutated at codon 12 (i.e. rasm12). This transfected clone presents at least 2 mutations, one concerning K-ras gene, and the other affecting an unrelated gene, responsible for the generation of a highly immunogenic, MHC class I restricted non-self peptide. The results indicate that cells of 'D' clone transfected with rasm12 were less immunogenic than cells of the same origin transfected with the vector alone. Moreover, rasm12)-transfected cells showed lower levels of H-2K(d) gene expression with respect to those detectable in control cells. In addition, in vivo and in vitro sensitization against 'D' clone carrying mutated ras did not result in a strong cytotoxic T lymphocyte response against rasm12-transfected non immunogenic L5178Y target cells. These preliminary results suggest that K-ras mutation could down-regulate the level of tumor immunogenicity, possibly acquired through a mutagenic process affecting other unrelated genes.
Testorelli C., B.S. (1997). Dacarbazine-induced immunogenicity of a murine leukemia is attenuated in cells transfected with mutated K-ras gene. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 16(1), 15-22.
|Tipologia:||Articolo su rivista|
|Citazione:||Testorelli C., B.S. (1997). Dacarbazine-induced immunogenicity of a murine leukemia is attenuated in cells transfected with mutated K-ras gene. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 16(1), 15-22.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/14|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1997|
|Titolo:||Dacarbazine-induced immunogenicity of a murine leukemia is attenuated in cells transfected with mutated K-ras gene|
|Autori:||Testorelli C., Bussini S., De Filippi R., Marelli O., Orlando L., Greiner J.W., Grohmann U., Tentori L., Giuliani A., Bonmassar E., Graziani G.|
|Appare nelle tipologie:||01 - Articolo su rivista|