Quantitation of Western blots from polyacrylamide gels is commonly accomplished by either visual inspection or densitometry. However, due to absorption of protein and staining reagents by the blotting membrane it is possible that visual inspection and reflectance densitometry will given anomalous results. In this paper we show such an example, and discuss how transmission densitometry can provide more accurate results.
Bolling, D., Brown, L., Aquino, A. (1989). Anomaly in western blot band intensities perceived by visual inspection. APPLIED AND THEORETICAL ELECTROPHORESIS, 1(3), 161-162.
Anomaly in western blot band intensities perceived by visual inspection
AQUINO, ANGELO
1989-01-01
Abstract
Quantitation of Western blots from polyacrylamide gels is commonly accomplished by either visual inspection or densitometry. However, due to absorption of protein and staining reagents by the blotting membrane it is possible that visual inspection and reflectance densitometry will given anomalous results. In this paper we show such an example, and discuss how transmission densitometry can provide more accurate results.File in questo prodotto:
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