Labeling the EBV membrane with octadecilrhodamine-b-chloride (R18) we were able to monitor spectrofluorometrically the early events of EBV fusion, under conditions in which we could affect PKC activity. Binding of EBV to Raji cells induces PKC translocation from the cytosol to the plasma membrane and 32P incorporation into its cellular receptor CR2. CR2 phosphorylation is completely inhibited when cells are preincubated with the PKC inhibitor calphostin c. This treatment also generates a strong inhibition of EBV fusion. Taken together this result suggests a key role of CR2 phosphorylation in the EBV entry into Raji cells.

Aquino, A., Lisi, A., Pozzi, D., Ravagnan, G., Grimaldi, S. (1993). EBV membrane receptor (CR2) is phosphorylated by protein kinase C (PKC) in the early stages of virus entry into lymphoblastoid cell line (Raji). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 196(2), 794-802 [10.1006/bbrc.1993.2319].

EBV membrane receptor (CR2) is phosphorylated by protein kinase C (PKC) in the early stages of virus entry into lymphoblastoid cell line (Raji)

AQUINO, ANGELO;
1993-01-01

Abstract

Labeling the EBV membrane with octadecilrhodamine-b-chloride (R18) we were able to monitor spectrofluorometrically the early events of EBV fusion, under conditions in which we could affect PKC activity. Binding of EBV to Raji cells induces PKC translocation from the cytosol to the plasma membrane and 32P incorporation into its cellular receptor CR2. CR2 phosphorylation is completely inhibited when cells are preincubated with the PKC inhibitor calphostin c. This treatment also generates a strong inhibition of EBV fusion. Taken together this result suggests a key role of CR2 phosphorylation in the EBV entry into Raji cells.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14
English
Con Impact Factor ISI
EPSTEIN-BARR-VIRUS; HUMAN LYMPHOCYTES-B; C3D RECEPTOR; EARLY ANTIGENS; IDENTIFICATION; CR-2; BINDING
Aquino, A., Lisi, A., Pozzi, D., Ravagnan, G., Grimaldi, S. (1993). EBV membrane receptor (CR2) is phosphorylated by protein kinase C (PKC) in the early stages of virus entry into lymphoblastoid cell line (Raji). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 196(2), 794-802 [10.1006/bbrc.1993.2319].
Aquino, A; Lisi, A; Pozzi, D; Ravagnan, G; Grimaldi, S
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/52724
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