The interaction of bovine pancreatic trypsin inhibitor (BPTI) and bovine tryptase, which are co-localized in the same granules of bovine mast cells, has been analyzed at 30°C in 0.1 M Tris-HCl, pH 8.0. The analysis has unravelled that the functional unit of bovine tryptase is formed of (at least) four binding sites for this inhibitor. These interaction sites display a simple binding behaviour for small inhibitors (and substrates), whereas heterogeneous properties have been observed in the binding of BPTI, Furthermore, in the presence of BPTI, a positive functional interaction can be detected among the binding sites also for a small synthetic inhibitor, like benzamidine. Such features indicate the existence of a complex functional interplay among the sites of the functional unit which is transmitted through the secondary specificity sites.

Fiorucci, L., Erba, F., Coletta, M., Ascoli, F. (1995). Evidence for multiple interacting binding sites in bovine tryptase. FEBS LETTERS, 363(2009/02/01 00:00:00.000), 81-84 [10.1016/0014-5793(95)00286-I].

Evidence for multiple interacting binding sites in bovine tryptase

FIORUCCI, LAURA;ERBA, FULVIO;COLETTA, MASSIMILIANO;
1995

Abstract

The interaction of bovine pancreatic trypsin inhibitor (BPTI) and bovine tryptase, which are co-localized in the same granules of bovine mast cells, has been analyzed at 30°C in 0.1 M Tris-HCl, pH 8.0. The analysis has unravelled that the functional unit of bovine tryptase is formed of (at least) four binding sites for this inhibitor. These interaction sites display a simple binding behaviour for small inhibitors (and substrates), whereas heterogeneous properties have been observed in the binding of BPTI, Furthermore, in the presence of BPTI, a positive functional interaction can be detected among the binding sites also for a small synthetic inhibitor, like benzamidine. Such features indicate the existence of a complex functional interplay among the sites of the functional unit which is transmitted through the secondary specificity sites.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
benzamidine; trypsin inhibitor; tryptase; animal cell; article; cattle; controlled study; enzyme binding; enzyme inhibition; mast cell; nonhuman; priority journal; Amino Acid Sequence; Animal; Aprotinin; Benzamidines; Binding Sites; Cattle; Cytoplasmic Granules; Hydrogen-Ion Concentration; Kinetics; Mast Cells; Molecular Sequence Data; Serine Endopeptidases; Support, Non-U.S. Gov't
Fiorucci, L., Erba, F., Coletta, M., Ascoli, F. (1995). Evidence for multiple interacting binding sites in bovine tryptase. FEBS LETTERS, 363(2009/02/01 00:00:00.000), 81-84 [10.1016/0014-5793(95)00286-I].
Fiorucci, L; Erba, F; Coletta, M; Ascoli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/52626
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