The interaction of bovine pancreatic trypsin inhibitor and bovine tryptase, isolated from liver capsule mast cells, was investigated. They form a complex in vitro with a K(i) of 5.6 nM at pH 8.0 and are localized within the mast cell granules, as shown by immunogold staining at the electron microscope level. In addition, double immunogold electron microscopy revealed that the inhibitor and the enzyme are present in the same granules, where they occur in clusters; this may be taken as an indication of their interaction in vivo and suggests a physiological role for bovine pancreatic trypsin inhibitor in the regulation of tryptase proteolytic activity.

Fiorucci, L., Erba, F., Falasca, L., Dini, L., Ascoli, F. (1995). Localization and interaction of bovine pancreatic trypsin inhibitor and tryptase in the granules of bovine mast cells. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1243(3), 407-413 [10.1016/0304-4165(94)00167-V].

Localization and interaction of bovine pancreatic trypsin inhibitor and tryptase in the granules of bovine mast cells

FIORUCCI, LAURA;ERBA, FULVIO;
1995-01-01

Abstract

The interaction of bovine pancreatic trypsin inhibitor and bovine tryptase, isolated from liver capsule mast cells, was investigated. They form a complex in vitro with a K(i) of 5.6 nM at pH 8.0 and are localized within the mast cell granules, as shown by immunogold staining at the electron microscope level. In addition, double immunogold electron microscopy revealed that the inhibitor and the enzyme are present in the same granules, where they occur in clusters; this may be taken as an indication of their interaction in vivo and suggests a physiological role for bovine pancreatic trypsin inhibitor in the regulation of tryptase proteolytic activity.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
BPTI; Localization; Mast cell; Serine protease; Tryptase
Fiorucci, L., Erba, F., Falasca, L., Dini, L., Ascoli, F. (1995). Localization and interaction of bovine pancreatic trypsin inhibitor and tryptase in the granules of bovine mast cells. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1243(3), 407-413 [10.1016/0304-4165(94)00167-V].
Fiorucci, L; Erba, F; Falasca, L; Dini, L; Ascoli, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/52624
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