Methods widely used to detect apoptosis do not allow us to easily distinguish between nuclei from viable or necrotic cells. Even if apoptosis and necrosis seem to occur as alternatives at the single cell level, they could be present simultaneously in a cell population much more frequently than expected. For this reason, attention was focused on attempting to recognize, by multiparameter flow cytometry, the characteristics of viable cells and of apoptotic or necrotic dead cells.
Matteucci, C., Grelli, S., De Smaele, E., Fontana, C., Mastino, A. (1999). Identification of nuclei from apoptotic, necrotic, and viable lymphoid cells by using multiparameter flow cytometry. CYTOMETRY, 35(2), 145-153.
Identification of nuclei from apoptotic, necrotic, and viable lymphoid cells by using multiparameter flow cytometry
MATTEUCCI, CLAUDIA;GRELLI, SANDRO;FONTANA, CARLA;MASTINO, ANTONIO
1999-02-01
Abstract
Methods widely used to detect apoptosis do not allow us to easily distinguish between nuclei from viable or necrotic cells. Even if apoptosis and necrosis seem to occur as alternatives at the single cell level, they could be present simultaneously in a cell population much more frequently than expected. For this reason, attention was focused on attempting to recognize, by multiparameter flow cytometry, the characteristics of viable cells and of apoptotic or necrotic dead cells.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.