We have examined the expression of 2 tumor-associated metalloproteinases, MMP-2 and MMP-9, in 48 primary cultures of prostatic carcinoma (PRCA) and 33 cultures of benign prostatic hyperplasia (BPH). PRCA cultures secrete significantly more MMP-9 than their benign counterparts. Secreted MMP-2 did not differ significantly in cultures but was lower in PRCA cultures. Two cultures of benign origin exhibited high MMP-9 secretion and growth patterns consistent with a malignancy. Both cases were followed and successively re-evaluated histologically and rediagnosed as organ-confined PRCA. MMP expression in culture may be of predictive value in the identification of incidental PRCA. MMP-9 secretion and its ratio with MMP-2 were highest in epithelial cultures from invasive, metastatic tumors when compared both to disease confined to prostate gland and to locally extensive disease. MMP-9 secretion was greatest also in cultures derived from tissues of high Gleason histological grade. Active MMP-9 species were detected in 15 cultures (31%) of PRCA. Active MMP-2. species were observed in cultures of both BPH and PRCA origin in almost the same amounts. Although average levels were not significantly different, as a ratio to preform species, a significant elevation was observed in cultures of PRCA origin. We propose, therefore, that an elevated expression of MMP-9 and a high ratio of MMP-9 to MMP-2 in short-term prostate epithelial cultures is of potential diagnostic and prognostic significance. (C) 1996 Wiley-Liss, Inc.
Festuccia, C., Bologna, M., Vicentini, C., Tacconelli, A., Miano, R., Violini, S., et al. (1996). Increased matrix metalloproteinase-9 secretion in short-term tissue cultures of prostatic tumor cells. INTERNATIONAL JOURNAL OF CANCER, 69(5), 386-393.
|Tipologia:||Articolo su rivista|
|Citazione:||Festuccia, C., Bologna, M., Vicentini, C., Tacconelli, A., Miano, R., Violini, S., et al. (1996). Increased matrix metalloproteinase-9 secretion in short-term tissue cultures of prostatic tumor cells. INTERNATIONAL JOURNAL OF CANCER, 69(5), 386-393.|
|IF:||Con Impact Factor ISI|
|Lingua:||English, Middle (1100-1500)|
|Settore Scientifico Disciplinare:||Settore MED/24 - Urologia|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/(SICI)1097-0215(19961021)69:5<386::AID-IJC6>3.0.CO;2-1|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1996|
|Titolo:||Increased matrix metalloproteinase-9 secretion in short-term tissue cultures of prostatic tumor cells|
|Autori:||Festuccia, C; Bologna, M; Vicentini, C; Tacconelli, A; Miano, R; Violini, S; Mackay, AR|
|Appare nelle tipologie:||01 - Articolo su rivista|