E2F1, a member of the E2F family of transcription factors, in addition to its established proliferative effect, has also been implicated in the induction of apoptosis through p53-dependent and p53-independent pathways. Several genes involved in the activation or execution of the apoptotic programme have recently been shown to be upregulated at the transcriptional level by E2F1 overexpression, including the genes encoding INK4a/ARF, Apaf-1, caspase 7 and p73 (refs 3-5). E2F1 is stabilized in response to DNA damage but it has not been established how this translates into the activation of specific subsets of E2F target genes. Here, we applied a chromatin immunoprecipitation approach to show that, in response to DNA damage, E2F1 is directed from cell cycle progression to apoptotic E2F target genes. We identify p73 as an important E2F1 apoptotic target gene in DNA damage response and we show that acetylation is required for E2F1 recruitment on the P1p73 promoter and for its transcriptional activation.

Pediconi, N., Ianari, A., Costanzo, A., Belloni, L., Gallo, R., Cimino, L., et al. (2003). Differential regulation of E2F1 apoptotic target genes in response to DNA damage. NATURE CELL BIOLOGY, 5(6), 552-558 [10.1038/ncb998].

Differential regulation of E2F1 apoptotic target genes in response to DNA damage

COSTANZO, ANTONIO;
2003-06-01

Abstract

E2F1, a member of the E2F family of transcription factors, in addition to its established proliferative effect, has also been implicated in the induction of apoptosis through p53-dependent and p53-independent pathways. Several genes involved in the activation or execution of the apoptotic programme have recently been shown to be upregulated at the transcriptional level by E2F1 overexpression, including the genes encoding INK4a/ARF, Apaf-1, caspase 7 and p73 (refs 3-5). E2F1 is stabilized in response to DNA damage but it has not been established how this translates into the activation of specific subsets of E2F target genes. Here, we applied a chromatin immunoprecipitation approach to show that, in response to DNA damage, E2F1 is directed from cell cycle progression to apoptotic E2F target genes. We identify p73 as an important E2F1 apoptotic target gene in DNA damage response and we show that acetylation is required for E2F1 recruitment on the P1p73 promoter and for its transcriptional activation.
giu-2003
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/35 - MALATTIE CUTANEE E VENEREE
English
Con Impact Factor ISI
Histones; Cell Cycle Proteins; Apoptosis; Chromatin; Genes, Tumor Suppressor; DNA Damage; Humans; DNA-Binding Proteins; Transcriptional Activation; Doxorubicin; E2F Transcription Factors; Tumor Suppressor Proteins; Fibroblasts; Gene Deletion; RNA, Messenger; Acetylation; Promoter Regions, Genetic; Tumor Cells, Cultured; Transcription Factors; Nuclear Proteins; E2F1 Transcription Factor; Genes, Reporter; Gene Expression Regulation; Etoposide
Pediconi, N., Ianari, A., Costanzo, A., Belloni, L., Gallo, R., Cimino, L., et al. (2003). Differential regulation of E2F1 apoptotic target genes in response to DNA damage. NATURE CELL BIOLOGY, 5(6), 552-558 [10.1038/ncb998].
Pediconi, N; Ianari, A; Costanzo, A; Belloni, L; Gallo, R; Cimino, L; Porcellini, A; Screpanti, I; Balsano, C; Alesse, E; Gulino, A; Levrero, M
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/52059
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact