This paper shows that cocaine amplifies Epstein-Barr virus (EBV) reactivation in Raji cells. Its effect on early viral protein synthesis was maximal when it was added with 12-O-tetradecanoyl phorbol-13-acetate (TPA) plus n-butyrate, but nil when added alone. The enhancing effect of cocaine on early replicative stages of latent EBV was associated with an increase of Ca2+ mobilization induced by the drug and with an induction of cellular protein phosphorylation in chemicals and cocaine-treated Raji cells. Cocaine also acted synergistically with TPA and n-butyrate to induce Z Epstein-Barr replication activator (ZEBRA), a nuclear phosphoprotein responsible for the activation of early viral gene expression. These findings provide the first evidence that cocaine may represent an important cofactor in the reactivation of early stages of latent EBV infection.

Di Francesco P., L.A. (1999). Cocaine potentiates the switch between latency and replication of Epstein-Barr virus in Raji cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 264(1), 33-36 [10.1006/bbrc.1999.1447].

Cocaine potentiates the switch between latency and replication of Epstein-Barr virus in Raji cells

GARACI, ENRICO
1999

Abstract

This paper shows that cocaine amplifies Epstein-Barr virus (EBV) reactivation in Raji cells. Its effect on early viral protein synthesis was maximal when it was added with 12-O-tetradecanoyl phorbol-13-acetate (TPA) plus n-butyrate, but nil when added alone. The enhancing effect of cocaine on early replicative stages of latent EBV was associated with an increase of Ca2+ mobilization induced by the drug and with an induction of cellular protein phosphorylation in chemicals and cocaine-treated Raji cells. Cocaine also acted synergistically with TPA and n-butyrate to induce Z Epstein-Barr replication activator (ZEBRA), a nuclear phosphoprotein responsible for the activation of early viral gene expression. These findings provide the first evidence that cocaine may represent an important cofactor in the reactivation of early stages of latent EBV infection.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - Microbiologia e Microbiologia Clinica
English
Con Impact Factor ISI
butyric acid; calcium ion; cell protein; cocaine; nuclear protein; phorbol 13 acetate 12 myristate; virus protein; animal cell; article; calcium mobilization; cell division; controlled study; epstein barr virus; gene expression; nonhuman; priority journal; protein induction; protein phosphorylation; protein synthesis; raji cell; virus gene; virus latency; Biological Transport; Butyrates; Calcium; Carcinogens; Cocaine; DNA-Binding Proteins; Drug Synergism; Herpesvirus 4, Human; Humans; Phosphorylation; Tetradecanoylphorbol Acetate; Trans-Activators; Tumor Cells, Cultured; Viral Proteins; Virus Latency; Virus Replication; Animalia; DNA viruses; Human herpesvirus 4
Di Francesco P., L.A. (1999). Cocaine potentiates the switch between latency and replication of Epstein-Barr virus in Raji cells. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 264(1), 33-36 [10.1006/bbrc.1999.1447].
Di Francesco, P; Lisi, A; Rieti, S; Manni, V; Grimaldi, S; Garaci, E
Articolo su rivista
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/52020
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