The sIgG(+) lymphoblastoid B cell line CESS spontaneously produces a high amount of NGF and expresses both high affinity (p140(Trk-A)) and low affinity (p75(NTR)) NGF receptors. Blocking NGF signals with neutralizing antibodies or specific Trk-A inhibitors induces a rapid phosphorylation of antiapoptotic Bcl-2 protein, followed by caspase activation, and apoptotic death of CESS cells. Bcl-2 phosphorylation in several sites within a approximate to 60 aa "loop" domain of protein is known to regulate its antiapoptotic function. Accordingly, CESS cells expressing the loop deletional mutant cDNA constructs Bcl-2 Delta 40-91 were completely resistant to apoptosis induced by NGF withdrawal, indicating that Bcl-2 phosphorylation is a critical event. NGF withdrawal induces p38 MAPK, but not JNK, activation in CESS cells, and SB203580, a specific inhibitor of p38 MAPK, is able to prevent both Bcl-2 phosphorylation and apoptosis, indicating that p38 MAPK is the enzyme responsible for these events. (C) 2000 Academic Press.
Rosini P., D.C.G. (2000). NGF withdrawal induces apoptosis in CESS B cell line through p38 MAPK activation and Bcl-2 phosphorylation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 278(3), 753-759.
|Tipologia:||Articolo su rivista|
|Citazione:||Rosini P., D.C.G. (2000). NGF withdrawal induces apoptosis in CESS B cell line through p38 MAPK activation and Bcl-2 phosphorylation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 278(3), 753-759.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/07 - Microbiologia e Microbiologia Clinica|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1006/bbrc.2000.3871|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||2000|
|Titolo:||NGF withdrawal induces apoptosis in CESS B cell line through p38 MAPK activation and Bcl-2 phosphorylation|
|Autori:||Rosini P., De Chiara G., Lucibello M., Garaci E., Cozzolino F., Torcia M.|
|Appare nelle tipologie:||01 - Articolo su rivista|