Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140(trk-A) and p75(NGFR) molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD ''virgin'' B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific lgG in mice immunized with tetanus toroid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.

Torcia M., B.L. (1996). Nerve growth factor is an autocrine survival factor for memory B lymphocytes. CELL, 85(3), 345-356 [10.1016/S0092-8674(00)81113-7].

Nerve growth factor is an autocrine survival factor for memory B lymphocytes

GARACI, ENRICO
1996

Abstract

Production of nerve growth factor (NGF) was assessed in cultures of human T and B lymphocytes and macrophages. NGF was constitutively produced by B cells only, which also expressed surface p140(trk-A) and p75(NGFR) molecules and hence efficiently bound and internalized the cytokine. Neutralization of endogenous NGF caused disappearance of Bcl-2 protein and apoptotic death of resting lymphocytes bearing surface IgG or IgA, a population comprising memory cells, while surface IgM/IgD ''virgin'' B lymphocytes were not affected. In vivo administration of neutralizing anti-NGF antibodies caused strong reduction in the titer of specific lgG in mice immunized with tetanus toroid, nitrophenol, or arsonate and reduced numbers of surface IgG or IgA B lymphocytes. Thus, NGF is an autocrine survival factor for memory B lymphocytes.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - Microbiologia e Microbiologia Clinica
English
Con Impact Factor ISI
nerve growth factor; animal cell; apoptosis; article; autocrine effect; b lymphocyte; cell survival; etiology; human; human cell; memory cell; mouse; nonhuman; priority journal; protein expression; Animals; Antibody Specificity; B-Lymphocyte Subsets; Cell Survival; Cells, Cultured; Female; Humans; Immunologic Memory; Immunophenotyping; Mice; Mice, Inbred BALB C; Nerve Growth Factors; Neutralization Tests; Proto-Oncogene Proteins; Receptor Protein-Tyrosine Kinases; Receptor, trkA; Receptors, Cell Surface; Receptors, Nerve Growth Factor
Torcia M., B.L. (1996). Nerve growth factor is an autocrine survival factor for memory B lymphocytes. CELL, 85(3), 345-356 [10.1016/S0092-8674(00)81113-7].
Torcia, M; Bracci Laudiero, L; Lucibello, M; Nencioni, L; Labardi, D; Rubartelli, A; Cozzolino, F; Aloe, L; Garaci, E
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/51978
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