We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700 bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidine-rich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.

Mariottini, P., Bagni, C., Francesconi, A., Cecconi, F., Serra, M.j., Chen, Q.m., et al. (1993). Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis. GENE, 132(2), 255-260 [10.1016/0378-1119(93)90204-G].

Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis

BAGNI, CLAUDIA;CECCONI, FRANCESCO;LORENI, FABRIZIO;AMALDI, FRANCESCO
1993-01-01

Abstract

We present here the cloning and the entire sequence of one of the two gene copies coding for ribosomal protein (r-protein) S8 in Xenopus laevis (corresponding to r-protein S7 in rat) and its flanking regions. The S8a gene contains seven exons and six introns for a total length of about 12700 bp coding for a mRNA of 663 nucleotides (nt) plus a poly(A) tail. Mapping of the 5' end of the gene has shown that the transcription start point is located in a pyrimidine-rich tract, as has been observed for all r-protein-encoding genes of X. laevis and other vertebrates so far characterized. A computer analysis of the S8a sequence has revealed the presence of a 220-nt sequence repeated, with some variations, once in each of the six introns. RNA analysis by hybridization with oligo probes specific for the two gene copies coding for r-protein S8 has demonstrated that the two of them are expressed at similar levels both in oocytes and in embryos.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
housekeeping gene; recombinant DNA; repeated sequences; ribosome
Mariottini, P., Bagni, C., Francesconi, A., Cecconi, F., Serra, M.j., Chen, Q.m., et al. (1993). Sequence of the gene coding for ribosomal protein S8 of Xenopus laevis. GENE, 132(2), 255-260 [10.1016/0378-1119(93)90204-G].
Mariottini, P; Bagni, C; Francesconi, A; Cecconi, F; Serra, Mj; Chen, Qm; Loreni, F; Annesi, F; Amaldi, F
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51830
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