The absence of the fragile X mental retardation protein (FMRP), encoded by the FMR1 gene, is responsible for pathologic manifestations in the Fragile X Syndrome, the most frequent cause of inherited mental retardation. FMRP is an RNA-binding protein associated with polysomes as part of a messenger ribonucleoprotein (mRNP) complex. Although its function is poorly understood, various observations suggest a role in local protein translation at neuronal dendrites and in dendritic spine maturation. We present here the identification of CYFIP1/2 (Cytoplasmic FMRP Interacting Proteins) as FMRP interactors. CYFIP1/2 share 88% amino acid sequence identity and represent the two members in humans of a highly conserved protein family. Remarkably, whereas CYFIP2 also interacts with the FMRP-related proteins FXR1P/2P, CYFIP1 interacts exclusively with FMRP, FMRP-CYFIP interaction involves the domain of FMRP also mediating homo- and heteromerization, thus suggesting a competition between interaction among the FXR proteins and interaction with CYFIP, CYFIP1/2 are proteins of unknown function, but CYFIP1 has recently been shown to interact with the small GTPase Rad, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and Rad localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts.

Schenck, A., Bardoni, B., Moro, A., Bagni, C., Mandel, J.l. (2001). A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 98(15), 8844-8849 [10.1073/pnas.151231598].

A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P

BAGNI, CLAUDIA;
2001-01-01

Abstract

The absence of the fragile X mental retardation protein (FMRP), encoded by the FMR1 gene, is responsible for pathologic manifestations in the Fragile X Syndrome, the most frequent cause of inherited mental retardation. FMRP is an RNA-binding protein associated with polysomes as part of a messenger ribonucleoprotein (mRNP) complex. Although its function is poorly understood, various observations suggest a role in local protein translation at neuronal dendrites and in dendritic spine maturation. We present here the identification of CYFIP1/2 (Cytoplasmic FMRP Interacting Proteins) as FMRP interactors. CYFIP1/2 share 88% amino acid sequence identity and represent the two members in humans of a highly conserved protein family. Remarkably, whereas CYFIP2 also interacts with the FMRP-related proteins FXR1P/2P, CYFIP1 interacts exclusively with FMRP, FMRP-CYFIP interaction involves the domain of FMRP also mediating homo- and heteromerization, thus suggesting a competition between interaction among the FXR proteins and interaction with CYFIP, CYFIP1/2 are proteins of unknown function, but CYFIP1 has recently been shown to interact with the small GTPase Rad, which is implicated in development and maintenance of neuronal structures. Consistent with FMRP and Rad localization in dendritic fine structures, CYFIP1/2 are present in synaptosomal extracts.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13
English
Con Impact Factor ISI
fragile x mental retardation protein; RNA binding protein; unclassified drug; amino acid sequence; animal tissue; article; fragile X syndrome; mental deficiency; mouse; nonhuman; priority journal; protein analysis; protein domain; protein family; protein interaction; sequence homology; synaptosome; Amino Acid Sequence; Animals; Base Sequence; Cell Extracts; Cell Fractionation; Cell Line; Cercopithecus aethiops; Conserved Sequence; COS Cells; DNA, Complementary; Exons; Fragile X Mental Retardation Protein; Gene Expression; Hela Cells; Humans; Molecular Sequence Data; Nerve Tissue Proteins; Proteins; Rabbits; Recombinant Fusion Proteins; RNA; RNA-Binding Proteins; Spodoptera; Animalia
Schenck, A., Bardoni, B., Moro, A., Bagni, C., Mandel, J.l. (2001). A highly conserved protein family interacting with the fragile X mental retardation protein (FMRP) and displaying selective interactions with FMRP-related proteins FXR1P and FXR2P. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 98(15), 8844-8849 [10.1073/pnas.151231598].
Schenck, A; Bardoni, B; Moro, A; Bagni, C; Mandel, Jl
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51768
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