Mast-cell growth factor (MGF) is encoded by the murine steel (Sl) locus and is a ligand for the tyrosine kinase receptor protein encoded by the proto-oncogene c-kit at the murine dominant white spotting (W) locus. Mutations at both these loci affect mast cells, primordial germ cells (PGCs), haemopoietic stem cells and melanocytes. In many Sl and W mutants, the rapid proliferation of PGC that normally occurs between day 7 and 13.5 of embryonic development fails to occur. As c-kit is expressed in PGCs while MGF is expressed in the surrounding mesenchyme, MGF might promote the proliferation of PGCs. Here we report that MGF is essential for PGC survival in culture, but does not stimulate PGC proliferation. Moreover, whereas both the transmembrane and soluble proteolytic cleavage forms of MGF stimulate mast-cell proliferation, soluble MGF has a relatively limited ability to support survival of PGCs in culture, thus explaining the sterility in mice carrying the steel-dickie (Sld) mutation, which encodes only a soluble form of MGF, and providing a functional role for a transmembrane growth factor.

DOLCI IANNINI, S., Williams, D., Ernst, M., Resnick, J., Brannan, C., Lock, L., et al. (1991). Requirement for mast cell growth factor for primordial germ cell survival in culture. NATURE, 352(6338), 809-811 [10.1038/352809a0].

Requirement for mast cell growth factor for primordial germ cell survival in culture

DOLCI IANNINI, SUSANNA;
1991-08-29

Abstract

Mast-cell growth factor (MGF) is encoded by the murine steel (Sl) locus and is a ligand for the tyrosine kinase receptor protein encoded by the proto-oncogene c-kit at the murine dominant white spotting (W) locus. Mutations at both these loci affect mast cells, primordial germ cells (PGCs), haemopoietic stem cells and melanocytes. In many Sl and W mutants, the rapid proliferation of PGC that normally occurs between day 7 and 13.5 of embryonic development fails to occur. As c-kit is expressed in PGCs while MGF is expressed in the surrounding mesenchyme, MGF might promote the proliferation of PGCs. Here we report that MGF is essential for PGC survival in culture, but does not stimulate PGC proliferation. Moreover, whereas both the transmembrane and soluble proteolytic cleavage forms of MGF stimulate mast-cell proliferation, soluble MGF has a relatively limited ability to support survival of PGCs in culture, thus explaining the sterility in mice carrying the steel-dickie (Sld) mutation, which encodes only a soluble form of MGF, and providing a functional role for a transmembrane growth factor.
29-ago-1991
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/16 - ANATOMIA UMANA
English
Animals; Recombinant Proteins; Proto-Oncogene Proteins c-kit; Gene Expression; Hematopoietic Cell Growth Factors; Mice; Stem Cell Factor; Cell Survival; Fibroblasts; RNA, Messenger; Germ Cells; Proto-Oncogene Proteins; Transfection; Embryo, Mammalian; Cell Line; Cell Division
DOLCI IANNINI, S., Williams, D., Ernst, M., Resnick, J., Brannan, C., Lock, L., et al. (1991). Requirement for mast cell growth factor for primordial germ cell survival in culture. NATURE, 352(6338), 809-811 [10.1038/352809a0].
DOLCI IANNINI, S; Williams, D; Ernst, M; Resnick, J; Brannan, C; Lock, L; Lyman, S; Boswell, H; Donovan, P
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51765
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 450
  • ???jsp.display-item.citation.isi??? 443
social impact