Follicle-stimulating hormone (FSH) and its intracellular mediator, cAMP, increase the mRNA levels for the Steel factor (SLF, the c-kit ligand) in cultured primary mouse Sertoli cells. The inductive effect of cAMP is more evident in cultures from 13-day-old animals than in cultures from 18-day-old animals. Analysis through the polymerase chain reaction (PCR) indicates that (Bu)2cAMP or FSH treatment increases the levels of the mRNAs for both the potentially soluble form and the transmembrane form of SLF in cultured Sertoli cells. The ratio between mRNAs encoding the potentially soluble form and the transmembrane form of SLF increases during postnatal testis development, and it is higher in cultured Sertoli cells with respect to total testis, suggesting that, under the in vitro conditions, SLF could be produced by Sertoli cells mainly as a soluble factor. Soluble recombinant SLF stimulates, in a dose-dependent fashion, thymidine incorporation in cultures of isolated germ cell populations enriched in the mitotic stages (spermatogonia), independently of the presence of serum, whereas cAMP analogs have no effect. Autoradiographic analysis shows that SLF selectively stimulates DNA synthesis in type A spermatogonia.

Rossi, P., DOLCI IANNINI, S., Albanesi, C., Grimaldi, P., Ricca, R., Geremia, R. (1993). Follicle-stimulating hormone induction of steel factor (SLF) mRNA in mouse Sertoli cells and stimulation of DNA synthesis in spermatogonia by soluble SLF. DEVELOPMENTAL BIOLOGY, 155(1), 68-74 [10.1006/dbio.1993.1007].

Follicle-stimulating hormone induction of steel factor (SLF) mRNA in mouse Sertoli cells and stimulation of DNA synthesis in spermatogonia by soluble SLF

ROSSI, PELLEGRINO;DOLCI IANNINI, SUSANNA;GRIMALDI, PAOLA;GEREMIA, RAFFAELE
1993-01-01

Abstract

Follicle-stimulating hormone (FSH) and its intracellular mediator, cAMP, increase the mRNA levels for the Steel factor (SLF, the c-kit ligand) in cultured primary mouse Sertoli cells. The inductive effect of cAMP is more evident in cultures from 13-day-old animals than in cultures from 18-day-old animals. Analysis through the polymerase chain reaction (PCR) indicates that (Bu)2cAMP or FSH treatment increases the levels of the mRNAs for both the potentially soluble form and the transmembrane form of SLF in cultured Sertoli cells. The ratio between mRNAs encoding the potentially soluble form and the transmembrane form of SLF increases during postnatal testis development, and it is higher in cultured Sertoli cells with respect to total testis, suggesting that, under the in vitro conditions, SLF could be produced by Sertoli cells mainly as a soluble factor. Soluble recombinant SLF stimulates, in a dose-dependent fashion, thymidine incorporation in cultures of isolated germ cell populations enriched in the mitotic stages (spermatogonia), independently of the presence of serum, whereas cAMP analogs have no effect. Autoradiographic analysis shows that SLF selectively stimulates DNA synthesis in type A spermatogonia.
gen-1993
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/16 - ANATOMIA UMANA
English
Con Impact Factor ISI
Animals; Oligodeoxyribonucleotides; Cyclic AMP; Gene Expression; Hematopoietic Cell Growth Factors; Transcription, Genetic; Mice; Stem Cell Factor; Spermatogenesis; Follicle Stimulating Hormone; RNA, Messenger; Base Sequence; Sertoli Cells; Alternative Splicing; Molecular Sequence Data; Male; Spermatogonia
Rossi, P., DOLCI IANNINI, S., Albanesi, C., Grimaldi, P., Ricca, R., Geremia, R. (1993). Follicle-stimulating hormone induction of steel factor (SLF) mRNA in mouse Sertoli cells and stimulation of DNA synthesis in spermatogonia by soluble SLF. DEVELOPMENTAL BIOLOGY, 155(1), 68-74 [10.1006/dbio.1993.1007].
Rossi, P; DOLCI IANNINI, S; Albanesi, C; Grimaldi, P; Ricca, R; Geremia, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51760
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