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EnglishWe describe a novel method for detecting micrometastasis in the blood stream of cancer patients based on RT-PCR amplification of tumor-associated carcinoembryonic antigen (CEA) mRNA. To increase sensitivity and specificity of RT-PCR, CEA transcript was selectively up-regulated in cancer cells by exposure of peripheral blood to non-toxic concentrations of staurosporine (ST). Thereafter, polyA(+) RNA was extracted from tumor cells captured by means of magnetic beads coated with a monoclonal antibody against a common human epithelial antigen. Finally, RNA was subjected to RT-PCR analysis of CEA transcript. Using this approach, we demonstrated an ST-mediated increase in CEA transcript in blood specimens collected from a patient with metastatic colon cancer before receiving treatment with 5-fluorouracil/leucovorin. After a few cycles of chemotherapy, CEA-positive tumor cells were no longer detected. Clinical follow-up of this patient indicated that treatment with chemotherapy induced a dramatic reduction in liver metastasis. Therefore, it can be hypothesized that lack of CEA transcript detection might be consistent with disappearance or at least marked reduction of circulating tumor cells.
| Tipologia: | Articolo su rivista |
| Citazione: | Aquino, A., Prete, S., Balduzzi, A., Fossile, E., Formica, V., Torino, F., et al. (2002). A novel method for monitoring response to chemotherapy based on the detection of circulating cancer cells: a case report. JOURNAL OF CHEMOTHERAPY, 14(4), 412-416. |
| IF: | Con Impact Factor ISI |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore BIO/14 Settore MED/06 - Oncologia Medica |
| Revisione (peer review): | Sì, ma tipo non specificato |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 2002 |
| Titolo: | A novel method for monitoring response to chemotherapy based on the detection of circulating cancer cells: a case report |
| Autori: | |
| Autori: | Aquino, A; Prete, S; Balduzzi, A; Fossile, E; Formica, V; Torino, F; Bonmassar, L; Di Giacomo, A; Cappelletti, D; Cardillo, A; Graziani, G |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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http://hdl.handle.net/2108/51741
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