The involvement of PKC in NG108-15 cell differentiation was investigated. Differentiation with dBcAMP was associated with a decrease in total cellular phorbol ester binding. The histone-directed PKC activity was decreased in the soluble fraction. Northern and Western blotting revealed the presence of only PKC alpha but not PKC beta and PKC gamma among the calcium-dependent isoforms. Differentiation induced a decrease of cytosolic PKC alpha immunoreactivity, with no changes of mRNA content or appearance of PKC beta and PKC gamma isoforms. The low levels of PKC alpha in the soluble fraction suggest that the mRNA for this species is less efficiently translated in differentiated NG108-15 cells. The data suggest that downregulation of PKC alpha protein and kinase activity are associated with induction of neuronal morphology in NG108-15 cells. (C) 1994 Academic Press, Inc.

Battaini, F.m., Garbillo, G., Bergamaschi, S., Parenti, M., Wetsel, W., Govoni, S., et al. (1994). REGULATION OF PROTEIN-KINASE-C IN NG108-15 CELL-DIFFERENTIATION. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 201(1), 135-142.

REGULATION OF PROTEIN-KINASE-C IN NG108-15 CELL-DIFFERENTIATION

BATTAINI, FIORENZO MARIA;TRABUCCHI, MARCO MARIO
1994-01-01

Abstract

The involvement of PKC in NG108-15 cell differentiation was investigated. Differentiation with dBcAMP was associated with a decrease in total cellular phorbol ester binding. The histone-directed PKC activity was decreased in the soluble fraction. Northern and Western blotting revealed the presence of only PKC alpha but not PKC beta and PKC gamma among the calcium-dependent isoforms. Differentiation induced a decrease of cytosolic PKC alpha immunoreactivity, with no changes of mRNA content or appearance of PKC beta and PKC gamma isoforms. The low levels of PKC alpha in the soluble fraction suggest that the mRNA for this species is less efficiently translated in differentiated NG108-15 cells. The data suggest that downregulation of PKC alpha protein and kinase activity are associated with induction of neuronal morphology in NG108-15 cells. (C) 1994 Academic Press, Inc.
1994
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14 - FARMACOLOGIA
English
Con Impact Factor ISI
NEURO-BLASTOMA CELLS; PHORBOL ESTER BINDING; NEUROBLASTOMA-CELLS; MESSENGER-RNAS; MORPHOLOGICAL-DIFFERENTIATION; EPSILON-ISOFORM; DOWN-REGULATION; RETINOIC ACID; HYBRID-CELLS; CYCLIC-AMP
8
Battaini, F.m., Garbillo, G., Bergamaschi, S., Parenti, M., Wetsel, W., Govoni, S., et al. (1994). REGULATION OF PROTEIN-KINASE-C IN NG108-15 CELL-DIFFERENTIATION. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 201(1), 135-142.
Battaini, Fm; Garbillo, G; Bergamaschi, S; Parenti, M; Wetsel, W; Govoni, S; Trabucchi, Mm
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51569
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