We have previously identified alterations of K+ channel function, IP3-mediated calcium release, and Cp20 (a memory-associated GTP binding protein) in fibroblasts from AD patients vs. controls. In the present study we introduce a scoring system based on these response alterations that integrates two or more alterations (and their degree) in AD vs. control fibroblasts. This scoring system generates an index that distinguishes AD patients from controls with both high specificity and sensitivity. We also show that low doses of bradykinin elicit intracellular calcium release almost exclusively in AD cell lines in an all or none fashion that provide a clear measurement of enhanced IP3-mediated function in AD vs. controls.

Hirashima, N., Etcheberrigaray, R., Bergamaschi, S., Racchi, M., Battaini, F.m., Binetti, G., et al. (1995). Calcium responses in human fibroblasts: a diagnostic molecular profile for Alzheimer's disease. NEUROBIOLOGY OF AGING, 17(4), 549-555.

Calcium responses in human fibroblasts: a diagnostic molecular profile for Alzheimer's disease

BATTAINI, FIORENZO MARIA;
1995-01-01

Abstract

We have previously identified alterations of K+ channel function, IP3-mediated calcium release, and Cp20 (a memory-associated GTP binding protein) in fibroblasts from AD patients vs. controls. In the present study we introduce a scoring system based on these response alterations that integrates two or more alterations (and their degree) in AD vs. control fibroblasts. This scoring system generates an index that distinguishes AD patients from controls with both high specificity and sensitivity. We also show that low doses of bradykinin elicit intracellular calcium release almost exclusively in AD cell lines in an all or none fashion that provide a clear measurement of enhanced IP3-mediated function in AD vs. controls.
1995
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14 - FARMACOLOGIA
English
Con Impact Factor ISI
Male; Aged, 80 and over; Cells, Cultured; Middle Aged; Bradykinin; Female; Aged; Alzheimer Disease; Calcium; Fibroblasts; Humans
Hirashima, N., Etcheberrigaray, R., Bergamaschi, S., Racchi, M., Battaini, F.m., Binetti, G., et al. (1995). Calcium responses in human fibroblasts: a diagnostic molecular profile for Alzheimer's disease. NEUROBIOLOGY OF AGING, 17(4), 549-555.
Hirashima, N; Etcheberrigaray, R; Bergamaschi, S; Racchi, M; Battaini, Fm; Binetti, G; Govoni, S; Alkon, D
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51558
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