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EnglishIn the present study we have examined the distribution of several isoforms of protein kinase C, a lipid-regulated serine/threonine kinase essential for signal transduction and cell regulation, in cultured human skin fibroblasts. By Western blot analysis we have detected the presence of at least three of the known protein kinase C isoforms. The calcium-dependent protein kinase C alpha was primarily associated with the cytosolic fraction. Three non-calcium-dependent isoforms, protein kinases C epsilon, C delta, and C zeta, were also detected. Protein kinases C zeta and C delta were present primarily in the cytosol, while protein kinase CE was associated primarily with the membrane fraction. Binding and activity studies were consistent with the pattern of expression and distribution defined by Western blot analysis. These results provide a useful frame of reference for the study of isoform-specific effects of protein kinase C in the regulation of cell growth and metabolism. (C) 1994 Academic Press, Inc.
| Tipologia: | Articolo su rivista |
| Citazione: | RACCHI, M., BERGAMASCHI, S., GOVONI, S., WETSEL, W.C., BIANCHETTI, A., BINETTI, G., et al. (1994). CHARACTERIZATION AND DISTRIBUTION OF PROTEIN-KINASE-C ISOFORMS IN HUMAN SKIN FIBROBLASTS. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 314(1), 107-111. |
| Altre informazioni significative: | 5 |
| IF: | Con Impact Factor ISI |
| Lingua: | English |
| Settore Scientifico Disciplinare: | Settore BIO/14 |
| Revisione (peer review): | Sì, ma tipo non specificato |
| Tipo: | Articolo |
| Rilevanza: | Rilevanza internazionale |
| Stato di pubblicazione: | Pubblicato |
| Data di pubblicazione: | 1994 |
| Titolo: | CHARACTERIZATION AND DISTRIBUTION OF PROTEIN-KINASE-C ISOFORMS IN HUMAN SKIN FIBROBLASTS |
| Autori: | |
| Autori: | RACCHI, M; BERGAMASCHI, S; GOVONI, S; WETSEL, WC; BIANCHETTI, A; BINETTI, G; BATTAINI, F; TRABUCCHI, M |
| Appare nelle tipologie: | 01 - Articolo su rivista |
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