In the present study we have examined the distribution of several isoforms of protein kinase C, a lipid-regulated serine/threonine kinase essential for signal transduction and cell regulation, in cultured human skin fibroblasts. By Western blot analysis we have detected the presence of at least three of the known protein kinase C isoforms. The calcium-dependent protein kinase C alpha was primarily associated with the cytosolic fraction. Three non-calcium-dependent isoforms, protein kinases C epsilon, C delta, and C zeta, were also detected. Protein kinases C zeta and C delta were present primarily in the cytosol, while protein kinase CE was associated primarily with the membrane fraction. Binding and activity studies were consistent with the pattern of expression and distribution defined by Western blot analysis. These results provide a useful frame of reference for the study of isoform-specific effects of protein kinase C in the regulation of cell growth and metabolism. (C) 1994 Academic Press, Inc.

Racchi, M., Bergamaschi, S., Govoni, S., Wetsel, W., Bianchetti, A., Binetti, G., et al. (1994). CHARACTERIZATION AND DISTRIBUTION OF PROTEIN-KINASE-C ISOFORMS IN HUMAN SKIN FIBROBLASTS. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 314(1), 107-111.

CHARACTERIZATION AND DISTRIBUTION OF PROTEIN-KINASE-C ISOFORMS IN HUMAN SKIN FIBROBLASTS

BATTAINI, FIORENZO MARIA;TRABUCCHI, MARCO MARIO
1994-01-01

Abstract

In the present study we have examined the distribution of several isoforms of protein kinase C, a lipid-regulated serine/threonine kinase essential for signal transduction and cell regulation, in cultured human skin fibroblasts. By Western blot analysis we have detected the presence of at least three of the known protein kinase C isoforms. The calcium-dependent protein kinase C alpha was primarily associated with the cytosolic fraction. Three non-calcium-dependent isoforms, protein kinases C epsilon, C delta, and C zeta, were also detected. Protein kinases C zeta and C delta were present primarily in the cytosol, while protein kinase CE was associated primarily with the membrane fraction. Binding and activity studies were consistent with the pattern of expression and distribution defined by Western blot analysis. These results provide a useful frame of reference for the study of isoform-specific effects of protein kinase C in the regulation of cell growth and metabolism. (C) 1994 Academic Press, Inc.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/14
English
Con Impact Factor ISI
PROTEIN KINASE C ISOFORMS; SIGNAL TRANSDUCTION; HUMAN SKIN FIBROBLASTS; SUBCELLULAR FRACTIONATION; PHORBOL ESTERS
5
Racchi, M., Bergamaschi, S., Govoni, S., Wetsel, W., Bianchetti, A., Binetti, G., et al. (1994). CHARACTERIZATION AND DISTRIBUTION OF PROTEIN-KINASE-C ISOFORMS IN HUMAN SKIN FIBROBLASTS. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 314(1), 107-111.
Racchi, M; Bergamaschi, S; Govoni, S; Wetsel, W; Bianchetti, A; Binetti, G; Battaini, Fm; Trabucchi, Mm
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51554
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