Testicular tumors in humans are reported to be significantly increasing in incidence. Embryo exposure to environmental estrogens has been proposed as one of the possible underlying causes. In mice, genetic, immunological, and experimental evidence suggest that germ cell testicular tumors may derive from primordial germ cells (PGCs), the embryonic precursors of gametes. Here we show that relatively high concentrations of estrogens stimulate mouse PGC growth in vitro through the somatic cells of the gonadal ridges. Moreover, we found that estrogens stimulate the transcription of the Steel gene and the production of c-Kit ligand in gonadal somatic cells, and that this growth factor is likely to be responsible for the observed stimulation of PGC growth via an Akt/PTEN pathway. Finally, we show that estrogen stimulation of gonadal somatic cells in culture, in combination with PTEN down-regulation in PGCs and the presence of leukemia inhibitory factor in the culture medium, result in high frequency of PGC transformation in tumorigenic cells. Based on these results, we present a novel experimental in vitro model for tumorigenic germ cell transformation and identify molecular pathways likely involved in development of germ cell tumors after estrogen exposure.

Moe Behrens, G., Klinger, F.g., Eskild, W., Grotmol, T., Haugen, T., DE FELICI, M. (2003). Akt/PTEN signaling mediates estrogen-dependent proliferation of primordial germ cells in vitro. MOLECULAR ENDOCRINOLOGY, 17(12), 2630-8 [10.1210/me.2003-0006].

Akt/PTEN signaling mediates estrogen-dependent proliferation of primordial germ cells in vitro

KLINGER, FRANCESCA GIOIA;DE FELICI, MASSIMO
2003-12-01

Abstract

Testicular tumors in humans are reported to be significantly increasing in incidence. Embryo exposure to environmental estrogens has been proposed as one of the possible underlying causes. In mice, genetic, immunological, and experimental evidence suggest that germ cell testicular tumors may derive from primordial germ cells (PGCs), the embryonic precursors of gametes. Here we show that relatively high concentrations of estrogens stimulate mouse PGC growth in vitro through the somatic cells of the gonadal ridges. Moreover, we found that estrogens stimulate the transcription of the Steel gene and the production of c-Kit ligand in gonadal somatic cells, and that this growth factor is likely to be responsible for the observed stimulation of PGC growth via an Akt/PTEN pathway. Finally, we show that estrogen stimulation of gonadal somatic cells in culture, in combination with PTEN down-regulation in PGCs and the presence of leukemia inhibitory factor in the culture medium, result in high frequency of PGC transformation in tumorigenic cells. Based on these results, we present a novel experimental in vitro model for tumorigenic germ cell transformation and identify molecular pathways likely involved in development of germ cell tumors after estrogen exposure.
dic-2003
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/17 - ISTOLOGIA
English
Con Impact Factor ISI
Germ Cells; Male; Protein-Serine-Threonine Kinases; Protein-Tyrosine Kinases; Testis; Female; Animals; Signal Transduction; Reverse Transcriptase Polymerase Chain Reaction; Mice; Pregnancy; Proto-Oncogene Proteins; Cell Division; Zearalenone; Genes, Reporter; Proto-Oncogene Proteins c-akt; Estradiol
Moe Behrens, G., Klinger, F.g., Eskild, W., Grotmol, T., Haugen, T., DE FELICI, M. (2003). Akt/PTEN signaling mediates estrogen-dependent proliferation of primordial germ cells in vitro. MOLECULAR ENDOCRINOLOGY, 17(12), 2630-8 [10.1210/me.2003-0006].
Moe Behrens, G; Klinger, Fg; Eskild, W; Grotmol, T; Haugen, T; DE FELICI, M
Articolo su rivista
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/51424
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 88
  • ???jsp.display-item.citation.isi??? 83
social impact