Cord blood has been shown to successfully reconstitute haematopoiesis following allogeneic transplantation in a variety of disorders. A major drawback of cord blood has been the risk of transfusion reaction secondary to ABO incompatibility and reduction in the stem cell pool if cord blood is manipulated to remove red cells. We report our experience on red blood cell depletion of cord blood (CB) with hydroxyethylstarch (HES) double sedimentation. The nucleated and mononucleated cell recovery passed from 78.4% at 90 min to 92.9% at 180 min and from 85% at 90 min to 96% at 180 min, respectively. The overall recovery of CCD34+ cells and of haemopoietic progenitors (CFU-GM) was 90.5% and 83.8%, respectively. The data indicate that HES double sedimentation is a simple and effective technique for cord blood manipulation, but further studies are necessary to evaluate the clonogenic progenitor recovery after thawing.

Adorno, G., Bruno, A., Caravita, T., Venditti, A., Ballatore, G., Santinelli, S., et al. (1998). Red blood cell depletion of cord blood using hydroxyethylstarch double sedimentation: analysis of 40 cases. CLINICAL AND LABORATORY HAEMATOLOGY, 20(6), 341-3.

Red blood cell depletion of cord blood using hydroxyethylstarch double sedimentation: analysis of 40 cases

ADORNO, GASPARE;VENDITTI, ADRIANO;POSTORINO, MASSIMILIANO;ARACO, PLACIDO;
1998-12

Abstract

Cord blood has been shown to successfully reconstitute haematopoiesis following allogeneic transplantation in a variety of disorders. A major drawback of cord blood has been the risk of transfusion reaction secondary to ABO incompatibility and reduction in the stem cell pool if cord blood is manipulated to remove red cells. We report our experience on red blood cell depletion of cord blood (CB) with hydroxyethylstarch (HES) double sedimentation. The nucleated and mononucleated cell recovery passed from 78.4% at 90 min to 92.9% at 180 min and from 85% at 90 min to 96% at 180 min, respectively. The overall recovery of CCD34+ cells and of haemopoietic progenitors (CFU-GM) was 90.5% and 83.8%, respectively. The data indicate that HES double sedimentation is a simple and effective technique for cord blood manipulation, but further studies are necessary to evaluate the clonogenic progenitor recovery after thawing.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/40 - Ginecologia e Ostetricia
English
Con Impact Factor ISI
Hetastarch; Fetal Blood; Female; Erythrocytes; Infant, Newborn; Hematocrit; Cell Separation; Humans
Adorno, G., Bruno, A., Caravita, T., Venditti, A., Ballatore, G., Santinelli, S., et al. (1998). Red blood cell depletion of cord blood using hydroxyethylstarch double sedimentation: analysis of 40 cases. CLINICAL AND LABORATORY HAEMATOLOGY, 20(6), 341-3.
Adorno, G; Bruno, A; Caravita, T; Venditti, A; Ballatore, G; Santinelli, S; Postorino, M; Monaco, I; Piazza, A; Calugi, A; Araco, P; Tribalto, M; Amadori, S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/51313
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