Identification of the motoneurons innervating the stapedius muscle in Gallus-gallus: a horseradish peroxidase study

The location of the stapedial motoneurons in Gallus gallus was investigated by means of the retrograde transport of HRP, injected into the stapedius muscle. The labeled neurons are located in both the ventral and dorsal divisions of the VII nerve nucleus, in a lateral and ventral position respectively, facing the superior olivary nucleus. The neurons are distributed in two size classes. The functional implications of these findings are discussed, in relation both to the absence of the acoustic stapedial reflex in birds and to the functional properties of the stapedius muscle.