The susceptibility of native, type-2 Cu-depleted and fully Cu-depleted ascorbate oxidase to thermal and chemical denaturation has been probed by differential scanning calorimetry, fluorimetry and circular dichroism. The data indicate that copper affects the stability, but not the protein conformation. The unfolding of ascorbate oxidase is characterized by a single endotherm. Calorimetric domains revealed by deconvolution are consistent with the domains identified by X-ray crystallography.

Savini I., D.S. (1990). The role of copper in the stability of ascorbate oxidase towards denaturing agents. EUROPEAN JOURNAL OF BIOCHEMISTRY, 190(3), 491-495.

The role of copper in the stability of ascorbate oxidase towards denaturing agents.

SAVINI, ISABELLA;AVIGLIANO, LUCIANA
1990

Abstract

The susceptibility of native, type-2 Cu-depleted and fully Cu-depleted ascorbate oxidase to thermal and chemical denaturation has been probed by differential scanning calorimetry, fluorimetry and circular dichroism. The data indicate that copper affects the stability, but not the protein conformation. The unfolding of ascorbate oxidase is characterized by a single endotherm. Calorimetric domains revealed by deconvolution are consistent with the domains identified by X-ray crystallography.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
ascorbate oxidase; copper; guanidine; guanidine derivative; oxidoreductase; article; circular dichroism; differential scanning calorimetry; enzyme stability; enzymology; heat; metabolism; plant; protein conformation; protein denaturation; spectrofluorometry; Ascorbate Oxidase; Calorimetry, Differential Scanning; Circular Dichroism; Copper; Enzyme Stability; Guanidine; Guanidines; Heat; Oxidoreductases; Plants; Protein Conformation; Protein Denaturation; Spectrometry, Fluorescence; Support, Non-U.S. Gov't
Savini I., D.S. (1990). The role of copper in the stability of ascorbate oxidase towards denaturing agents. EUROPEAN JOURNAL OF BIOCHEMISTRY, 190(3), 491-495.
Savini, I; D'Alessio, S; Giartosio, A; Morpurgo, L; Avigliano, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/51167
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