A very pure ascorbate oxidase solution was obtained by dissolving a crystalline sample of the enzyme. The ratio between 280 and 610 nm absorbancies was 22.5. It contained 8.0 ± 0.2 Cu ions, 50% EPR detectable, per dimeric molecule (140,000 M.W.) with a molar extinction coefficient of 10,000 cm-1 at 610 nm. Two Cu ions were removed by treatment with N,N-diethyldithiocarbamate. The optical blue absorption band was unaffected, while two EPR detectable Cu ions were lost, with disappearance of the type 2 Cu signal. It is concluded that native ascorbate oxidase contains two type 1, two type 1, two type 2, and four type 3 Cu ions. © 1988 Academic Press, Inc.

Morpurgo, L., Savini, I., Gatti, G., Bolognesi, M., Avigliano, L. (1988). Reassessment of copper stoichiometry in ascorbate oxidase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 152(2), 623-628.

Reassessment of copper stoichiometry in ascorbate oxidase

SAVINI, ISABELLA;AVIGLIANO, LUCIANA
1988

Abstract

A very pure ascorbate oxidase solution was obtained by dissolving a crystalline sample of the enzyme. The ratio between 280 and 610 nm absorbancies was 22.5. It contained 8.0 ± 0.2 Cu ions, 50% EPR detectable, per dimeric molecule (140,000 M.W.) with a molar extinction coefficient of 10,000 cm-1 at 610 nm. Two Cu ions were removed by treatment with N,N-diethyldithiocarbamate. The optical blue absorption band was unaffected, while two EPR detectable Cu ions were lost, with disappearance of the type 2 Cu signal. It is concluded that native ascorbate oxidase contains two type 1, two type 1, two type 2, and four type 3 Cu ions. © 1988 Academic Press, Inc.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
ascorbate oxidase; copper; copper protein; metalloprotein; oxidoreductase; vegetable protein; article; crystallization; electron spin resonance; spectrophotometry; Ascorbate Oxidase; Copper; Crystallization; Electron Spin Resonance Spectroscopy; Metalloproteins; Oxidoreductases; Spectrophotometry; Support, Non-U.S. Gov't; Vegetable Proteins
Morpurgo, L., Savini, I., Gatti, G., Bolognesi, M., Avigliano, L. (1988). Reassessment of copper stoichiometry in ascorbate oxidase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 152(2), 623-628.
Morpurgo, L; Savini, I; Gatti, G; Bolognesi, M; Avigliano, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/51166
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