We have investigated the enzymatic reduction and accumulation of vitamin C in HaCaT epithelial cells. The subcellular localization and the activities of ascorbyl free radical reductase and dehydroascorbate reductase showed that mitochondrial, microsomal and plasma membranes fractions express high levels of ascorbyl free radical reductase activity, whereas dehydroascorbate reductase activity was found at low levels only in the post microsomal supernatant. We have also investigated cell proliferation and vitamin C accumulation induced by ascorbic acid 2-phosphate. This derivative caused no inhibition of cell growth, was uptaken from the extracellular medium and accumulated as ascorbic acid in mM concentrations. These results show that HaCaT cells possess very efficient systems to maintain high levels of both intracellular and extracellular ascorbic acid. The regeneration and uptake of ascorbic acid from extracellular medium contributes to the intracellular antioxidant capacity, as evaluated by 2',7'-dihydrodichlorofluorescein staining. Consequently, cells became more resistant to free radical generation and cell death induced by UV-B irradiation. (C) 1999 Elsevier Science Inc.
Savini I., D.I. (1999). Ascorbic acid maintenance in HaCaT cells prevents radical formation and apoptosis by UV-B. FREE RADICAL BIOLOGY & MEDICINE, 26(2009/10/09 00:00:00.000), 1172-1180.
|Tipologia:||Articolo su rivista|
|Citazione:||Savini I., D.I. (1999). Ascorbic acid maintenance in HaCaT cells prevents radical formation and apoptosis by UV-B. FREE RADICAL BIOLOGY & MEDICINE, 26(2009/10/09 00:00:00.000), 1172-1180.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore MED/49 - Scienze Tecniche Dietetiche Applicate|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/S0891-5849(98)00311-6|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||1999|
|Titolo:||Ascorbic acid maintenance in HaCaT cells prevents radical formation and apoptosis by UV-B|
|Autori:||Savini I., D'Angelo I., Ranalli M., Melino G., Avigliano L.|
|Appare nelle tipologie:||01 - Articolo su rivista|