Vitamin C plays an important role in neutralizing toxic free radicals formed during oxidative metabolism or UV exposure of human skin. This study was performed to investigate the mechanisms that regulate the homoeostasis of vitamin C in HaCaT cells by identifying the events involved in the transport and in the reduction of dehydroascorbic acid. Dehydroascorbic acid accumulated to a greater extent and faster compared with ascorbic acid; its transport appeared to be mediated by hexose transporters and was entirely distinct from ascorbic acid transport. Dehydroascorbate reductase activity was unaffected by glutathione depletion, although it was sensitive to thiol protein reagents. These observations, as well as the subcellular distribution of this enzymic activity and the cofactor specificity, indicate that thioredoxin reductase and lipoamide dehydrogenase play an important role in this reduction process. HaCaT cells were able to enhance their dehydroascorbic acid reductase activity in response to oxidative stress.

Savini I., D.S. (2000). Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent. BIOCHEMICAL JOURNAL, 345(3), 665-672.

Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent

SAVINI, ISABELLA;AVIGLIANO, LUCIANA
2000

Abstract

Vitamin C plays an important role in neutralizing toxic free radicals formed during oxidative metabolism or UV exposure of human skin. This study was performed to investigate the mechanisms that regulate the homoeostasis of vitamin C in HaCaT cells by identifying the events involved in the transport and in the reduction of dehydroascorbic acid. Dehydroascorbic acid accumulated to a greater extent and faster compared with ascorbic acid; its transport appeared to be mediated by hexose transporters and was entirely distinct from ascorbic acid transport. Dehydroascorbate reductase activity was unaffected by glutathione depletion, although it was sensitive to thiol protein reagents. These observations, as well as the subcellular distribution of this enzymic activity and the cofactor specificity, indicate that thioredoxin reductase and lipoamide dehydrogenase play an important role in this reduction process. HaCaT cells were able to enhance their dehydroascorbic acid reductase activity in response to oxidative stress.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/49 - Scienze Tecniche Dietetiche Applicate
eng
Con Impact Factor ISI
Dehydroascorbic acid reductase; Lipoamide dehydrogenase; Thioredoxin reductase
Savini I., D.S. (2000). Dehydroascorbic acid uptake in a human keratinocyte cell line (HaCaT) is glutathione-independent. BIOCHEMICAL JOURNAL, 345(3), 665-672.
Savini, I; Duflot, S; Avigliano, L
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/51152
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