BACKGROUND: Combination of age at diagnosis, stage and MYCN amplification stratifies neuroblastoma into low-risk and high-risk. We aimed to establish whether a microRNA (miRNA) signature could be associated with prognosis in both groups. METHODS: Microarray expression profiling of human miRNAs and quantitative reverse-transcriptase PCR of selected miRNAs were performed on a preliminary cohort of 13 patients. Results were validated on an independent cohort of 214 patients. The relationship between miRNA expression and the overall or disease-free survival was analysed on the total cohort of 227 patients using the log-rank test and the multivariable Cox proportional hazard model. RESULTS: A total of 15 of 17 miRNAs that discriminated high-risk from low-risk neuroblastoma belonged to the imprinted human 14q32.31 miRNA cluster and two, miR-487b and miR-410, were significantly downregulated in the high-risk group. Multivariable analyses showed miR-487b expression as associated with overall survival and disease-free survival in the whole cohort, independently of clinical covariates. Moreover, miR-487b and miR-410 expression was significantly associated with disease-free survival of the non-MYCN-amplified favourable neuroblastoma: localised (stage 1, 2 and 3) and stage 4 of infant <18 months. CONCLUSION: Expression of miR-487b and miR-410 shows predictive value beyond the classical high-/low-risk stratification and is a biomarker of relapse in favourable neuroblastoma.
Gattolliat, C.H., Thomas, L., Ciafrè, S.A., Meurice, G., Le Teuff, G., Job, B., et al. (2011). Expression of miR-487b and miR-410 encoded by 14q32.31 locus is a prognostic marker in neuroblastoma. BRITISH JOURNAL OF CANCER, 105(9), 1352-1361.
|Tipologia:||Articolo su rivista|
|Citazione:||Gattolliat, C.H., Thomas, L., Ciafrè, S.A., Meurice, G., Le Teuff, G., Job, B., et al. (2011). Expression of miR-487b and miR-410 encoded by 14q32.31 locus is a prognostic marker in neuroblastoma. BRITISH JOURNAL OF CANCER, 105(9), 1352-1361.|
|IF:||Con Impact Factor ISI|
|Settore Scientifico Disciplinare:||Settore BIO/13|
|Revisione (peer review):||Sì, ma tipo non specificato|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/bjc.2011.388|
|Stato di pubblicazione:||Pubblicato|
|Data di pubblicazione:||25-ott-2011|
|Titolo:||Expression of miR-487b and miR-410 encoded by 14q32.31 locus is a prognostic marker in neuroblastoma.|
|Autori:||Gattolliat, CH; Thomas, L; Ciafrè, SA; Meurice, G; Le Teuff, G; Job, B; Richon, C; Combaret, V; Dessen, P; Valteau-Couanet, D; May, E; Busson, P; Douc-Rasy, S; Bénard, J;|
|Appare nelle tipologie:||01 - Articolo su rivista|