We have studied the patterns of interleukin-2 (IL-2), IL-4 and interferon-gamma (IFN-gamma) co-expression displayed by individual splenic CD4(+) and CD8(+) T cells in response to influenza virus immunization. Unseparated spleen cells obtained from mice intraperitoneally (i.p.) injected with A/PR8 (H1N1) influenza virus (PR8) were cultured for 24 hr in the presence of ultraviolet-inactivated PR8. As controls, cultures of both naive spleen cells stimulated with PR8 or of immune cells lacking the: inactivated virus were used. The frequencies of CD4(+) and CD8(+) T cells expressing IL-2, IL-4 and IFN-gamma were determined by three-colour flow cytometric analysis of fixed and saponin-permeabilized cells fluorescent-stained for either CD4 or CD8 surface molecules and for one of the following combinations of two intracellular cytokines: IL-2/IL-4, IL-2/IFN-gamma and IL-4/IFN-gamma. The results showed that immunization with influenza virus induces in both CD4(+) and CD8(+) T cells a heterogeneity of cytokine response patterns that do not follow the type 1/type 2 polarized response model, but with substantial differences between the two populations. In fact, the analysis of the phenotypes of virus-immune CD8(+) T cells revealed similar significant proportions of cells either expressing any one of the three cytokines or co-expressing combinations of them (i.e. IL-4/IL-2, IL-4/IFN-gamma and IL-2/IFN-gamma), whereas immune CD4(+) T cells were seen to express almost exclusively a single cytokine per cell. The observed patterns of cytokine production suggest that influenza virus immunization induces the expression of a type 0 cytokine pattern at both population and single cell levels in CD8(+) T cells and exclusively at the population level in CD4(+) T cells.

Falchetti, R., Di Francesco, P., Lanzilli, G., Gaziano, R., Casalinuovo, I.a., Palamara, A.t., et al. (1998). Determination of cytokine co-expression in individual splenic CD4(+) and CD8(+) T cells from influenza virus-immune mice. IMMUNOLOGY, 95(3), 346-351 [10.1046/j.1365-2567.1998.00608.x].

Determination of cytokine co-expression in individual splenic CD4(+) and CD8(+) T cells from influenza virus-immune mice

GAZIANO, ROBERTA;CASALINUOVO, IDA ANTONIA;
1998-01-01

Abstract

We have studied the patterns of interleukin-2 (IL-2), IL-4 and interferon-gamma (IFN-gamma) co-expression displayed by individual splenic CD4(+) and CD8(+) T cells in response to influenza virus immunization. Unseparated spleen cells obtained from mice intraperitoneally (i.p.) injected with A/PR8 (H1N1) influenza virus (PR8) were cultured for 24 hr in the presence of ultraviolet-inactivated PR8. As controls, cultures of both naive spleen cells stimulated with PR8 or of immune cells lacking the: inactivated virus were used. The frequencies of CD4(+) and CD8(+) T cells expressing IL-2, IL-4 and IFN-gamma were determined by three-colour flow cytometric analysis of fixed and saponin-permeabilized cells fluorescent-stained for either CD4 or CD8 surface molecules and for one of the following combinations of two intracellular cytokines: IL-2/IL-4, IL-2/IFN-gamma and IL-4/IFN-gamma. The results showed that immunization with influenza virus induces in both CD4(+) and CD8(+) T cells a heterogeneity of cytokine response patterns that do not follow the type 1/type 2 polarized response model, but with substantial differences between the two populations. In fact, the analysis of the phenotypes of virus-immune CD8(+) T cells revealed similar significant proportions of cells either expressing any one of the three cytokines or co-expressing combinations of them (i.e. IL-4/IL-2, IL-4/IFN-gamma and IL-2/IFN-gamma), whereas immune CD4(+) T cells were seen to express almost exclusively a single cytokine per cell. The observed patterns of cytokine production suggest that influenza virus immunization induces the expression of a type 0 cytokine pattern at both population and single cell levels in CD8(+) T cells and exclusively at the population level in CD4(+) T cells.
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore MED/07 - Microbiologia e Microbiologia Clinica
English
Con Impact Factor ISI
cd4 antigen; cd8 antigen; cytokine; gamma interferon; interleukin 2; interleukin 4; animal cell; animal experiment; article; controlled study; flow cytometry; helper cell; influenza vaccination; influenza virus; male; mouse; nonhuman; priority journal; protein expression; spleen cell; suppressor cell; t lymphocyte; virus inactivation; Animals; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Cell Culture Techniques; Cytokines; Flow Cytometry; Immunization; Influenza A virus; Influenza Vaccines; Interferon Type II; Interleukin-2; Interleukin-4; Male; Mice; Mice, Inbred BALB C; Spleen
Falchetti, R., Di Francesco, P., Lanzilli, G., Gaziano, R., Casalinuovo, I.a., Palamara, A.t., et al. (1998). Determination of cytokine co-expression in individual splenic CD4(+) and CD8(+) T cells from influenza virus-immune mice. IMMUNOLOGY, 95(3), 346-351 [10.1046/j.1365-2567.1998.00608.x].
Falchetti, R; Di Francesco, P; Lanzilli, G; Gaziano, R; Casalinuovo, Ia; Palamara, At; Ravagnan, G; Garaci, E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/50579
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