The human glioblastoma cell line LI showed morphological features typical of its neuroectodermal origin. Cells were positive by immunofluorescence to GFAP, MHC class II, and L1 determinants. Cytogenetic analysis showed the presence of a modal chromosome number of 63, ranging from 58 to 69 chromosomes (DNA index was 1.6). Northern blot analysis demonstrated the presence of mRNA transcripts specific for transglutaminase C (type II or "tissue"), growth-hormone releasing-hormone (GHRH), insulin-like growth factor II (IGF-II), and proopiomelanocortin (POMC). The GHRH mRNA was present in two different sizes, one similar to the normal hypothalamic species of 0.75 kb, whilst the second species was a large transcript of approximately 10 kb size. Treatment with 5-mu-M retinoic acid or 5 mM alpha-difluoromethylornithine for 5 days sharply reduced the growth rate and also induced modulation of the ultrastructure and antigenic profile. This cell line may be useful to study glial differentiation and the relationship of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal tumours.

Savarese, A., Annicchiarico Petruzzelli, M., Citro, G., Zupi, G., Spagnoli, L.g., Colantoni, A., et al. (1992). Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones. EXPERIMENTAL BRAIN RESEARCH, 89(2), 408-414.

Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones

VERNOLE, PATRIZIA;MELINO, GENNARO
1992-01-01

Abstract

The human glioblastoma cell line LI showed morphological features typical of its neuroectodermal origin. Cells were positive by immunofluorescence to GFAP, MHC class II, and L1 determinants. Cytogenetic analysis showed the presence of a modal chromosome number of 63, ranging from 58 to 69 chromosomes (DNA index was 1.6). Northern blot analysis demonstrated the presence of mRNA transcripts specific for transglutaminase C (type II or "tissue"), growth-hormone releasing-hormone (GHRH), insulin-like growth factor II (IGF-II), and proopiomelanocortin (POMC). The GHRH mRNA was present in two different sizes, one similar to the normal hypothalamic species of 0.75 kb, whilst the second species was a large transcript of approximately 10 kb size. Treatment with 5-mu-M retinoic acid or 5 mM alpha-difluoromethylornithine for 5 days sharply reduced the growth rate and also induced modulation of the ultrastructure and antigenic profile. This cell line may be useful to study glial differentiation and the relationship of GHRH, IGF-II and POMC expression with differentiation in neuroectodermal tumours.
1992
Pubblicato
Rilevanza nazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13 - BIOLOGIA APPLICATA
English
Con Impact Factor ISI
eflornithine; glial fibrillary acidic protein; growth hormone releasing factor; hypophysis hormone; hypothalamus hormone; major histocompatibility antigen class 2; messenger rna; proopiomelanocortin; protein glutamine gamma glutamyltransferase; retinoic acid; adult; antigen expression; article; cell differentiation; cell ultrastructure; chromosome analysis; chromosome number; controlled study; glioblastoma; growth rate; human; human cell; male; northern blotting; priority journal; tumor cell line; Blotting, Northern; Cytogenetics; DNA, Neoplasm; Glioma; Hormones; Human; Hypothalamus; Insulin-Like Growth Factor II; Male; Microscopy, Electron; Middle Age; Nervous System Neoplasms; Phenotype; Pituitary Hormones; Pro-Opiomelanocortin; Somatotropin-Releasing Hormone; Support, Non-U.S. Gov't; Tretinoin; Tumor Cells, Cultured
Savarese, A., Annicchiarico Petruzzelli, M., Citro, G., Zupi, G., Spagnoli, L.g., Colantoni, A., et al. (1992). Characterisation of a human glioblastoma cell line (LI) expressing hypothalamic and pituitary hormones. EXPERIMENTAL BRAIN RESEARCH, 89(2), 408-414.
Savarese, A; Annicchiarico Petruzzelli, M; Citro, G; Zupi, G; Spagnoli, Lg; Colantoni, A; Vernole, P; Stephanou, A; Knight, Ra; Guerrieri, P; Melino, ...espandi
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/50287
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