A technique of in situ hybridization on metaphases of chromosomes by a digoxigenin-labeled probe is described. This technique was able to detect single DNA sequences of 2 and 7 kilobases. The results obtained were compared with those of a biotin streptavidin alkaline phosphatase-based detection system. The digoxigenin method was at least as efficient and sensitive as the biotin-streptavidin method.
Vernole, P. (1990). Digoxigenin-labeled probes can detect single-copy genes in human metaphase chromosomes. BIOTECHNIQUES, 9(2), 200-204.
Digoxigenin-labeled probes can detect single-copy genes in human metaphase chromosomes
VERNOLE, PATRIZIA
1990-08-01
Abstract
A technique of in situ hybridization on metaphases of chromosomes by a digoxigenin-labeled probe is described. This technique was able to detect single DNA sequences of 2 and 7 kilobases. The results obtained were compared with those of a biotin streptavidin alkaline phosphatase-based detection system. The digoxigenin method was at least as efficient and sensitive as the biotin-streptavidin method.File in questo prodotto:
Non ci sono file associati a questo prodotto.
Questo articolo è pubblicato sotto una Licenza Licenza Creative Commons
