Bleomycin induces DNA and chromosome breakage. The differential sensitivity to the drug has been used in vitro to identify individuals at high risk of developing tumours. However, there are limited reports on the ability of bleomycin to induce apoptosis. In this study we tested induction of apoptosis in human peripheral lymphocytes by bleomycin at different concentrations and different culture times using various parameters, such as nuclear fragmentation and DNA fragmentation, evaluated either in situ with terminal transferase and labelled nucleotides (TUNEL) or by flow cytometry analysis. We demonstrate that bleomycin induces apoptosis without previous permeabilization of the cell membrane. Cell death occurs mainly by apoptosis and not by necrosis, with significant alteration of membrane lipoperoxidation (evaluated by luminescence).

Vernole, P., Tedeschi, B., Caporossi, D., Maccarrone, M., Melino, G., Annicchiarico Petruzzelli, M. (1998). Induction of apoptosis by bleomycin in resting and cycling human lymphocytes. MUTAGENESIS, 13(3), 209-215 [10.1093/mutage/13.3.209].

Induction of apoptosis by bleomycin in resting and cycling human lymphocytes

VERNOLE, PATRIZIA;MELINO, GENNARO;
1998-01-01

Abstract

Bleomycin induces DNA and chromosome breakage. The differential sensitivity to the drug has been used in vitro to identify individuals at high risk of developing tumours. However, there are limited reports on the ability of bleomycin to induce apoptosis. In this study we tested induction of apoptosis in human peripheral lymphocytes by bleomycin at different concentrations and different culture times using various parameters, such as nuclear fragmentation and DNA fragmentation, evaluated either in situ with terminal transferase and labelled nucleotides (TUNEL) or by flow cytometry analysis. We demonstrate that bleomycin induces apoptosis without previous permeabilization of the cell membrane. Cell death occurs mainly by apoptosis and not by necrosis, with significant alteration of membrane lipoperoxidation (evaluated by luminescence).
1998
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/13 - BIOLOGIA APPLICATA
English
Con Impact Factor ISI
bleomycin; dna fragment; apoptosis; article; cell death; cell membrane; cell nucleus; chromosome breakage; dna strand breakage; flow cytometry; human; human cell; normal human; peripheral lymphocyte; priority journal; Adult; Apoptosis; Bleomycin; Cell Cycle; Cells, Cultured; DNA Fragmentation; Humans; Interphase; Luminescent Measurements; Lymphocytes; Middle Aged; Necrosis
Vernole, P., Tedeschi, B., Caporossi, D., Maccarrone, M., Melino, G., Annicchiarico Petruzzelli, M. (1998). Induction of apoptosis by bleomycin in resting and cycling human lymphocytes. MUTAGENESIS, 13(3), 209-215 [10.1093/mutage/13.3.209].
Vernole, P; Tedeschi, B; Caporossi, D; Maccarrone, M; Melino, G; Annicchiarico Petruzzelli, M
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/50025
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