Direct (unmediated) electrochemistry of horseradish peroxidase (HRP) immobilized within a polymeric film is investigated at a pyrolytic graphite electrode by de cyclic voltammetry, in the absence and in the presence of hydrogen peroxide. Under the latter condition. a reduction wave centered at approx. -280 mV (vs. SCE) is observed, the intensity of which is strictly dependent on the hydrogen peroxide concentration. This permits a voltammetric investigation of the electrocatalytic reduction of hydrogen peroxide. Flow and flow-injection measurements carried out at constant potential under the same conditions, support voltammetric data. The suitability of the immobilized HRP-based electrodic system to monitor the presence of important analytes, such as glucose or choline, in solution, is also discussed. To this issue, suitable amounts of HRP and glucose oxidase (or, in turn, choline oxidase) were simultaneously entrapped in the polymer. The results obtained are of potential value for basic and applied biochemistry and represent a first step for construction of a mediator-free (third-generation) biosensor which may find application in the biosensoristic area. (C) 1998 Elsevier Science S.A. All rights reserved.

Ferri, T., Poscia, A., Santucci, R. (1998). Direct electrochemistry of membrane-entrapped horseradish peroxidase. Part II: Amperometric detection of hydrogen peroxide. BIOELECTROCHEMISTRY AND BIOENERGETICS, 45(2), 221-226 [10.1016/S0302-4598(98)00102-0].

Direct electrochemistry of membrane-entrapped horseradish peroxidase. Part II: Amperometric detection of hydrogen peroxide

SANTUCCI, ROBERTO
1998-01-01

Abstract

Direct (unmediated) electrochemistry of horseradish peroxidase (HRP) immobilized within a polymeric film is investigated at a pyrolytic graphite electrode by de cyclic voltammetry, in the absence and in the presence of hydrogen peroxide. Under the latter condition. a reduction wave centered at approx. -280 mV (vs. SCE) is observed, the intensity of which is strictly dependent on the hydrogen peroxide concentration. This permits a voltammetric investigation of the electrocatalytic reduction of hydrogen peroxide. Flow and flow-injection measurements carried out at constant potential under the same conditions, support voltammetric data. The suitability of the immobilized HRP-based electrodic system to monitor the presence of important analytes, such as glucose or choline, in solution, is also discussed. To this issue, suitable amounts of HRP and glucose oxidase (or, in turn, choline oxidase) were simultaneously entrapped in the polymer. The results obtained are of potential value for basic and applied biochemistry and represent a first step for construction of a mediator-free (third-generation) biosensor which may find application in the biosensoristic area. (C) 1998 Elsevier Science S.A. All rights reserved.
1998
Pubblicato
Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10 - BIOCHIMICA
English
Con Impact Factor ISI
Biosensor; Chemically modified electrode; Cyclic voltammetry; Horseradish peroxidase; Protein entrapment
Ferri, T., Poscia, A., Santucci, R. (1998). Direct electrochemistry of membrane-entrapped horseradish peroxidase. Part II: Amperometric detection of hydrogen peroxide. BIOELECTROCHEMISTRY AND BIOENERGETICS, 45(2), 221-226 [10.1016/S0302-4598(98)00102-0].
Ferri, T; Poscia, A; Santucci, R
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2108/49992
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