The effect of glycerol on the structure of cytochrome c was investigated by circular dichroism, absorbance and EPR spectroscopy. The results obtained show that an increasing concentration of the organic solvent (70-99.2%, v/v) in aqueous polyalcohol mixtures converts native cytochrome c into a new, low spin form through a fully reversible, two-state transition. The glycerol-stabilized form (that we call here the G state) retains native-like amounts of α-helix structure while rigid tertiary structure and native Fe(III)-Met(80) axial bond are lost. Analysis of data suggests a molten globule character of the G state; support to this view is afforded by the striking similarities between the spectroscopic (and, thus, structural) properties of the G state with those of the acidic molten globule of the protein (A state).
Santucci R., P.F. (1999). Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol. BIOCHIMIE, 81(7), 745-750.
Tipologia: | Articolo su rivista |
Citazione: | Santucci R., P.F. (1999). Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol. BIOCHIMIE, 81(7), 745-750. |
IF: | Con Impact Factor ISI |
Lingua: | English |
Settore Scientifico Disciplinare: | Settore BIO/10 |
Revisione (peer review): | Sì, ma tipo non specificato |
Tipo: | Articolo |
Rilevanza: | Rilevanza internazionale |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1016/S0300-9084(99)80132-1 |
Stato di pubblicazione: | Pubblicato |
Data di pubblicazione: | 1999 |
Titolo: | Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol |
Autori: | |
Autori: | Santucci R., Polizio F., Desideri A. |
Appare nelle tipologie: | 01 - Articolo su rivista |