The effect of glycerol on the structure of cytochrome c was investigated by circular dichroism, absorbance and EPR spectroscopy. The results obtained show that an increasing concentration of the organic solvent (70-99.2%, v/v) in aqueous polyalcohol mixtures converts native cytochrome c into a new, low spin form through a fully reversible, two-state transition. The glycerol-stabilized form (that we call here the G state) retains native-like amounts of α-helix structure while rigid tertiary structure and native Fe(III)-Met(80) axial bond are lost. Analysis of data suggests a molten globule character of the G state; support to this view is afforded by the striking similarities between the spectroscopic (and, thus, structural) properties of the G state with those of the acidic molten globule of the protein (A state).

Santucci R., P.F. (1999). Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol. BIOCHIMIE, 81(7), 745-750 [10.1016/S0300-9084(99)80132-1].

Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol

SANTUCCI, ROBERTO;POLIZIO, FRANCESCA;DESIDERI, ALESSANDRO
1999

Abstract

The effect of glycerol on the structure of cytochrome c was investigated by circular dichroism, absorbance and EPR spectroscopy. The results obtained show that an increasing concentration of the organic solvent (70-99.2%, v/v) in aqueous polyalcohol mixtures converts native cytochrome c into a new, low spin form through a fully reversible, two-state transition. The glycerol-stabilized form (that we call here the G state) retains native-like amounts of α-helix structure while rigid tertiary structure and native Fe(III)-Met(80) axial bond are lost. Analysis of data suggests a molten globule character of the G state; support to this view is afforded by the striking similarities between the spectroscopic (and, thus, structural) properties of the G state with those of the acidic molten globule of the protein (A state).
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Rilevanza internazionale
Articolo
Sì, ma tipo non specificato
Settore BIO/10
English
Con Impact Factor ISI
A-state; Circular dichroism; Cytochrome c; Glycerol; Molten globule
Santucci R., P.F. (1999). Formation of a molten-globule-like state of cytochrome c induced by high concentrations of glycerol. BIOCHIMIE, 81(7), 745-750 [10.1016/S0300-9084(99)80132-1].
Santucci, R; Polizio, F; Desideri, A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2108/49991
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